MasteringBiology - Genetic Engineering
Complete Study Guide Test.
in a gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel. -
CORRECT ANSWER negative...positive
what information can not be obtained from the sequence of a gene? - CORRECT ANSWER whether the
gene is methylated
what is the polymerase chain reaction (PCR)? - CORRECT ANSWER a method to amplify a fragment of
DNA
true or false? comparison of the sequences of the same gene across species can give some insight into
the existence of a common ancestor with that gene. - CORRECT ANSWER true
true or false? the Taq enzyme is a type of DNA polymerase that allows researchers to separate the DNA
strands during the annealing step of the PCR cycle without destroying the polymerase. - CORRECT
ANSWER false
how many DNA molecules would there be after four rounds of PCR if the initial reaction mixture
contained two molecules? - CORRECT ANSWER 32
during which step in the PCR cycle are nucleotides used? - CORRECT ANSWER extension
during which step in the PCR cycle do primers form bonds with a single-stranded template? - CORRECT
ANSWER annealing
which enzymes will produce a DNA fragment that contains the entire vgp gene and has "sticky ends?" -
CORRECT ANSWER HindIII and BamHI
Complete Study Guide Test.
in a gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel. -
CORRECT ANSWER negative...positive
what information can not be obtained from the sequence of a gene? - CORRECT ANSWER whether the
gene is methylated
what is the polymerase chain reaction (PCR)? - CORRECT ANSWER a method to amplify a fragment of
DNA
true or false? comparison of the sequences of the same gene across species can give some insight into
the existence of a common ancestor with that gene. - CORRECT ANSWER true
true or false? the Taq enzyme is a type of DNA polymerase that allows researchers to separate the DNA
strands during the annealing step of the PCR cycle without destroying the polymerase. - CORRECT
ANSWER false
how many DNA molecules would there be after four rounds of PCR if the initial reaction mixture
contained two molecules? - CORRECT ANSWER 32
during which step in the PCR cycle are nucleotides used? - CORRECT ANSWER extension
during which step in the PCR cycle do primers form bonds with a single-stranded template? - CORRECT
ANSWER annealing
which enzymes will produce a DNA fragment that contains the entire vgp gene and has "sticky ends?" -
CORRECT ANSWER HindIII and BamHI
