(Fortis) BIO101 - Molecular Biology - Latest Midterm Exam Review

BIO 101




Molecular Biology




LATEST MIDTERM EXAM
REVIEW




©Fortis 2024/2025

,1. Which technique is most commonly used for amplifying small
segments of DNA?
a) Southern blot
b) Northern blot
c) Polymerase Chain Reaction (PCR)
d) Western blot
Answer: c) Polymerase Chain Reaction (PCR)
Rationale: PCR is the gold standard technique for amplifying
small segments of DNA due to its precision and efficiency.


2. Which of the following methods is used to analyze gene
expression by measuring mRNA levels?
a) ELISA
b) Western Blot
c) qRT-PCR
d) Southern blot
Answer: c) qRT-PCR
Rationale: Quantitative real-time PCR (qRT-PCR) measures
mRNA levels, providing insights into gene expression.


©Fortis 2024/2025

, 3. What is the central principle of the Central Dogma of Molecular
Biology?
a) RNA -> DNA -> Protein
b) DNA -> RNA -> Protein
c) DNA -> Protein -> RNA
d) RNA -> Protein -> DNA
Answer: b) DNA -> RNA -> Protein
Rationale: The Central Dogma of Molecular Biology describes
the flow of genetic information from DNA to RNA to Protein.


4. CRISPR-Cas9 technology is primarily used for:
a) DNA sequencing
b) Gene editing
c) Protein synthesis
d) RNA interference
Answer: b) Gene editing
Rationale: CRISPR-Cas9 allows for precise editing of the
genome by creating cuts at specific DNA sites.


5. The primary function of ribosomes in cells is to:
a) Replicate DNA

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