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RNA INTERFERENCE AND SIRNAS QUESTIONS AND ANSWERS

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RNA INTERFERENCE AND SIRNAS QUESTIONS AND ANSWERS

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RNA INTERFERENCE AND SIRNAS
QUESTIONS AND ANSWERS
Non-coding sRNAs s- sans✔✔no sORF

ORF s- sans✔✔Open sReading sFrame
needs sstart sand sstop scodon
produce sprotein sof sat sleast s50 samino sacids

Small snon-coding sRNAs s- sans✔✔Shorter sthan s30nt
regulate sanother sRNA svia sbase-pairing

Small snon-coding sRNAs sclasses s- sans✔✔siRNAs
miRNAs
piRNAs

siRNAs s- sans✔✔short sinterfering sRNAs
originate sfrom sexternal ssource sto ssilence sinvading sRNA

endo-siRNAs s- sans✔✔siRNAs sexpressed sby sthe sgenome

Exogenous s- sans✔✔external

miRNAs s- sans✔✔microRNAs s
encoded sby sgenome sto sprevent stranslation sof san smRNA

piRNAs s- sans✔✔piwi-interacting sRNAs
encoded sby sgenome sto srepress stransposons

Function sof sNoncoding sRNAs s- sans✔✔Immune ssystem
degrade sviral sRNA
Guard sgenome sfrom stransposable selements

(scientists sharness sthis sto sknock sout sor sknock sdown sgenes)

Small sRNAs sact sby sbase spairing s- sans✔✔siRNAs suse sPERFECT sbase spairing sto
srecognize starget



RNA sinterference sdiscovery s- sans✔✔Fire's sLab snoticed sthat sdsRNA scould spost
stranscriptionally ssilence sgene sexpression sin sc. selegans

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