Phlebotomy Order of Draw Study NHCO
Exam Questions And Answers
Lab responsible for blood cultures? - correct answer✅Microbiology
Additive in blood cultures? - correct answer✅Sodium polyanethole
sulfonate- SPS (Nutrient Broth)
How and what do you clean for blood culture? - correct
answer✅ChloraPrep (not alc) at injection site and wipe down the tops
of collection bottles
What order do you fill blood culture bottles? - correct answer✅Aerobic
first and then anaerobic (butterfly tubing contains air, hence why
aerobic is first)
What does STAT mean? - correct answer✅Test must be carried out
immediately
T/F) Blood cultures are usually ordered STAT or as timed specimens -
correct answer✅True
What is the purpose of sodium polyanethole sulfonate (SPS)? - correct
answer✅To promote rapid growth of pathogenic/infectious
microorganisms in a blood sample (for identification)- blood culture
bottles
,Phlebotomy Order of Draw Study NHCO
Exam Questions And Answers
What tubes come before and after blood cultures? - correct
answer✅No tubes before, discard tube after (clear or glass red top).
What needle should you use for a blood culture? - correct answer✅Use
a winged infusion set (butterfly) to prevent nutrient broth introduction
to blood stream (may differ by employer)
Which red top tube (glass/plastic) do you use as a discard? - correct
answer✅Glass (glass red tops contain no additives)
What is a discard tube used for? - correct answer✅Used to prevent
thrombin and other cells from polluting the samples drawn in following
tubes.
What tubes come before and after a discard tube? - correct
answer✅Blood culture bottles before, light blue top tube after
What tubes come before and after a light blue top? - correct
answer✅Discard tube before (clear, red glass), royal blue with red
stripe after
T/F) the royal blue with red stripe tube has no additives - correct
answer✅True
, Phlebotomy Order of Draw Study NHCO
Exam Questions And Answers
Does the royal blue red stripe tube yield serum or plasma? - correct
answer✅Serum
What is the difference between a glass red top tube (no additive) and a
royal blue red stripe tube? - correct answer✅The royal blue red stripe
tube contains no nickel
What is the difference between a purple top tube and a royal blue
lavender/blue stripe tube? - correct answer✅The royal blue
lavender/blue stripe tube contains no nickel
What 2 tests are royal blue tubes used for? (Red stripe or lavender/blue
stripe) - correct answer✅Lead (Pb) poisoning, Heavy metal toxicology
Lab responsible for royal blue tubes? (Red stripe or lavender/blue
stripe) - correct answer✅Toxicology
How many times should you invert royal blue tube? (Red stripe or
lavender/blue stripe) - correct answer✅8 times (but you will invert all
tubes 8-10 times)
What tubes come before and after royal blue red stripe tube? - correct
answer✅Light blue before, plastic red top after
Exam Questions And Answers
Lab responsible for blood cultures? - correct answer✅Microbiology
Additive in blood cultures? - correct answer✅Sodium polyanethole
sulfonate- SPS (Nutrient Broth)
How and what do you clean for blood culture? - correct
answer✅ChloraPrep (not alc) at injection site and wipe down the tops
of collection bottles
What order do you fill blood culture bottles? - correct answer✅Aerobic
first and then anaerobic (butterfly tubing contains air, hence why
aerobic is first)
What does STAT mean? - correct answer✅Test must be carried out
immediately
T/F) Blood cultures are usually ordered STAT or as timed specimens -
correct answer✅True
What is the purpose of sodium polyanethole sulfonate (SPS)? - correct
answer✅To promote rapid growth of pathogenic/infectious
microorganisms in a blood sample (for identification)- blood culture
bottles
,Phlebotomy Order of Draw Study NHCO
Exam Questions And Answers
What tubes come before and after blood cultures? - correct
answer✅No tubes before, discard tube after (clear or glass red top).
What needle should you use for a blood culture? - correct answer✅Use
a winged infusion set (butterfly) to prevent nutrient broth introduction
to blood stream (may differ by employer)
Which red top tube (glass/plastic) do you use as a discard? - correct
answer✅Glass (glass red tops contain no additives)
What is a discard tube used for? - correct answer✅Used to prevent
thrombin and other cells from polluting the samples drawn in following
tubes.
What tubes come before and after a discard tube? - correct
answer✅Blood culture bottles before, light blue top tube after
What tubes come before and after a light blue top? - correct
answer✅Discard tube before (clear, red glass), royal blue with red
stripe after
T/F) the royal blue with red stripe tube has no additives - correct
answer✅True
, Phlebotomy Order of Draw Study NHCO
Exam Questions And Answers
Does the royal blue red stripe tube yield serum or plasma? - correct
answer✅Serum
What is the difference between a glass red top tube (no additive) and a
royal blue red stripe tube? - correct answer✅The royal blue red stripe
tube contains no nickel
What is the difference between a purple top tube and a royal blue
lavender/blue stripe tube? - correct answer✅The royal blue
lavender/blue stripe tube contains no nickel
What 2 tests are royal blue tubes used for? (Red stripe or lavender/blue
stripe) - correct answer✅Lead (Pb) poisoning, Heavy metal toxicology
Lab responsible for royal blue tubes? (Red stripe or lavender/blue
stripe) - correct answer✅Toxicology
How many times should you invert royal blue tube? (Red stripe or
lavender/blue stripe) - correct answer✅8 times (but you will invert all
tubes 8-10 times)
What tubes come before and after royal blue red stripe tube? - correct
answer✅Light blue before, plastic red top after
