MIC 102 Midterm 2
Study Guide (solved)
pyrimidine - answer nucleotide bases with one ring
purine - answer nucleotide bases with two rings
3' OH end (5' phosphate group of new nucleotide) - answer To which
end of the growing strand are nucleotides added by DNAP?
oriC sequence - answer Where does replication begin?
DNA polymerase III - answer Polyermase that runs replication 5' to 3'
DNAa - answer _______ binds to DNA at oriC sequence and "melts"
DNA in order to open up and expose strands
oriC - answer region of DNA that is AT rich
,SSBps - answer molecules that bind to DNA and hold it open after they
have been "melted" by DNAa
AT bonds - answer nucleotide basepairs with 2 hydrogen bonds
CG - answer nucleotide basepairs with 3 hydrogen bonds
dnaC - answer loading factor for helicase DNAb
dnaB - answer helicase that recruits replisome proteins once loaded by
dnaC
dnag - answer RNA polymerase that makes primers needed to start
synthesis
dnaa - answer very important protein for controlling initiation; oriC has
multiple binding sites that all need to be filled before cell can initiate
-need to time replication so there's enough ATP and also cell cycle
growth
SeqA - answer protein that sequesters the origin: keeps replication from
happening by blocking dnaA from binding to newly methlyated strands
-may also associate new origin to spot on cell mebrane
, true - answer Newly synthesized strands are not methylated
immediately and are hemi-methylated, which causes SeqA to have a
high affinity and blocks dnaA binding site to initiate replication (T/F)
Elongation - answer DNAP 3 moves quickly on leading strand and adds
bp to 3' end
RNAase H - answer removes RNA primer (made by DNAg) from lagging
strand
DNA P1 - answer fills in nucleotides on lagging strand between
nucleotides proofreading as it goes
DNA ligase - answer fills in gaps between Okazaki fragments
replisome - answer protein machinery that controls replication made up
of the proteins
DNA gyrase - answer as replisome moves forward, DNA ahead of it
turns into positive super coils and this protein is needed to unwind and
untangle the chromosome
ciproflaxin - answer broad spectrum antibiotic that targets DNA gryase
Study Guide (solved)
pyrimidine - answer nucleotide bases with one ring
purine - answer nucleotide bases with two rings
3' OH end (5' phosphate group of new nucleotide) - answer To which
end of the growing strand are nucleotides added by DNAP?
oriC sequence - answer Where does replication begin?
DNA polymerase III - answer Polyermase that runs replication 5' to 3'
DNAa - answer _______ binds to DNA at oriC sequence and "melts"
DNA in order to open up and expose strands
oriC - answer region of DNA that is AT rich
,SSBps - answer molecules that bind to DNA and hold it open after they
have been "melted" by DNAa
AT bonds - answer nucleotide basepairs with 2 hydrogen bonds
CG - answer nucleotide basepairs with 3 hydrogen bonds
dnaC - answer loading factor for helicase DNAb
dnaB - answer helicase that recruits replisome proteins once loaded by
dnaC
dnag - answer RNA polymerase that makes primers needed to start
synthesis
dnaa - answer very important protein for controlling initiation; oriC has
multiple binding sites that all need to be filled before cell can initiate
-need to time replication so there's enough ATP and also cell cycle
growth
SeqA - answer protein that sequesters the origin: keeps replication from
happening by blocking dnaA from binding to newly methlyated strands
-may also associate new origin to spot on cell mebrane
, true - answer Newly synthesized strands are not methylated
immediately and are hemi-methylated, which causes SeqA to have a
high affinity and blocks dnaA binding site to initiate replication (T/F)
Elongation - answer DNAP 3 moves quickly on leading strand and adds
bp to 3' end
RNAase H - answer removes RNA primer (made by DNAg) from lagging
strand
DNA P1 - answer fills in nucleotides on lagging strand between
nucleotides proofreading as it goes
DNA ligase - answer fills in gaps between Okazaki fragments
replisome - answer protein machinery that controls replication made up
of the proteins
DNA gyrase - answer as replisome moves forward, DNA ahead of it
turns into positive super coils and this protein is needed to unwind and
untangle the chromosome
ciproflaxin - answer broad spectrum antibiotic that targets DNA gryase
