BCMB 406B - Lab 2 exam

Glycoside hydrolases - Hydrolyze the glycosidic bond between 2 or more carbohydrates or carbohydrate-protein bonds Carbohydrate Binding Modules - - Amino acid sequence within a carbohydrate-active enzyme with a discreet fold that has carbohydrate binding activity - Maintains the interaction of the enzyme with its substrate Vector NTI Advance - - Allows you to plan your experiment and ensure that it results in the desired product Benefits of in silico - - Predicts restriction fragment sizes for WT and mutant CBM construct - Verifying that the desired mutation is present without additional mutations - Can make sure that your gene is cloned in frame with the fusion partner so that the whole gene is expressed Novagen's pET System - - Gene of interest is cloned under control of the T7 promoter - Expression of the gene is induced by providing T7 RNA polymerase - Target genes may be transcriptionally maintained in a silent state when not induced - Target protein can be induced by IPTG or lactose when transferred to an expression host