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MLT ASCP EXAM PREPARATORY QUESTIONS AND ANSWERS

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MLT ASCP EXAM PREPARATORY QUESTIONS AND ANSWERS A chemical reaction that is utilized by many different analytical methods involves: Fluorometry Glucose oxidase The reduction of NAD to NADH UV spectrophotometry -Answer-The reduction of NAD to NADH A nitrate test is performed on a glucose no fermenter. When the nitrate reagents were added, no color change occurs. When zinc dust was added, no color develops. How should this test be reported? Positive for nitrite and negative for molecular nitrogen Negative for molecular nitrogen and negative for nitrate production Positive for molecular nitrogen and negative for nitrite production Negative for nitrate and negative for nitrite reduction -Answer-Positive for molecular nitrogen and negative for nitrite production A patient has a history of repeated spontaneous abortion. Coagulation studies reveal an elevated APTT, normal PT, normal platelet function, and normal thrombin time. Schistocytes were seen on the peripheral blood smear. Factor VIII assay Mixing studies with factor-deficient plasmas Antinuclear antibody test Platelet neutralization test -Answer-Platelet neutralization test A patient, who recently received a transfusion of three units of red cells, is DAT-positive with an IgG specificity. What would be the best method to identify the patient's phenotype? Adsorption and elution Type and Screen Polymerase chain reaction-based assay Antigen typing -Answer-Polymerase chain reaction-based assay A reaction is in ____________ kinetics when the reaction rate depends only on enzyme concentration. Zero-order First-order Second-order Mixed-order -Answer-Zero-order Acetest® can be used to test for ketones in: A. urine B. urine and serum C. urine, serum and whole blood D. urine, serum, whole blood and CSF -Answer-C. urine, serum and whole blood All of the following are associated with Hgb C disease, EXCEPT? Hgb C crystal Target cells Lysine substituted for glutamic acid at the sixth position of the beta chain Fast mobility of Hgb C at pH 8.6 -Answer-Fast mobility of Hgb C at pH 8.6 An electrophoretic separation of lactate dehydrogenase isoenzyme that demonstrates elevation in LD-1 greater than LD-2 could be indicative of: A normal LD isoenzyme pattern Hemolysis Pancreatitis Hepatic injury -Answer-Hemolysis Antibodies directed against granulocyte antigens have been implicated for all of the following EXCEPT? Febrile transfusion reactions Acute hemolytic transfusion reactions Transfusion-related acute lung injury (TRALI) Neonatal alloimmune neutropenia (NAN) -Answer-Acute hemolytic transfusion reactions Approximately how many doses are required to obtain a steady-state oscillation allowing for peak and trough levels to be evaluated? 1 to 2 3 to 4 5 to 7 10 -Answer-5 to 7 Based on the results below, what is the most likely organism? Gram stain: Positive cocci Catalase: Positive Coagulase: Negative Bacitracin susceptibility: Resistant Novobiocin susceptibility: Resistant

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A chemical reaction that is utilized by many different analytical methods involves:

Fluorometry
Glucose oxidase
The reduction of NAD to NADH
UV spectrophotometry -Answer-The reduction of NAD to NADH

A nitrate test is performed on a glucose no fermenter. When the nitrate reagents were
added, no color change occurs. When zinc dust was added, no color develops. How
should this test be reported?

Positive for nitrite and negative for molecular nitrogen
Negative for molecular nitrogen and negative for nitrate production
Positive for molecular nitrogen and negative for nitrite production
Negative for nitrate and negative for nitrite reduction -Answer-Positive for molecular
nitrogen and negative for nitrite production

A patient has a history of repeated spontaneous abortion. Coagulation studies reveal an
elevated APTT, normal PT, normal platelet function, and normal thrombin time.
Schistocytes were seen on the peripheral blood smear.

Factor VIII assay
Mixing studies with factor-deficient plasmas
Antinuclear antibody test
Platelet neutralization test -Answer-Platelet neutralization test

A patient, who recently received a transfusion of three units of red cells, is DAT-positive
with an IgG specificity. What would be the best method to identify the patient's
phenotype?

Adsorption and elution
Type and Screen
Polymerase chain reaction-based assay
Antigen typing -Answer-Polymerase chain reaction-based assay

A reaction is in ____________ kinetics when the reaction rate depends only on enzyme
concentration.

Zero-order
First-order
Second-order
Mixed-order -Answer-Zero-order

, Acetest® can be used to test for ketones in:

A. urine
B. urine and serum
C. urine, serum and whole blood
D. urine, serum, whole blood and CSF -Answer-C. urine, serum and whole blood

All of the following are associated with Hgb C disease, EXCEPT?

Hgb C crystal
Target cells
Lysine substituted for glutamic acid at the sixth position of the beta chain
Fast mobility of Hgb C at pH 8.6 -Answer-Fast mobility of Hgb C at pH 8.6

An electrophoretic separation of lactate dehydrogenase isoenzyme that demonstrates
elevation in LD-1 greater than LD-2 could be indicative of:

A normal LD isoenzyme pattern
Hemolysis
Pancreatitis
Hepatic injury -Answer-Hemolysis

Antibodies directed against granulocyte antigens have been implicated for all of the
following EXCEPT?

Febrile transfusion reactions
Acute hemolytic transfusion reactions
Transfusion-related acute lung injury (TRALI)
Neonatal alloimmune neutropenia (NAN) -Answer-Acute hemolytic transfusion reactions

Approximately how many doses are required to obtain a steady-state oscillation
allowing for peak and trough levels to be evaluated?

1 to 2
3 to 4
5 to 7
> 10 -Answer-5 to 7

Based on the results below, what is the most likely organism?
Gram stain: Positive cocci
Catalase: Positive
Coagulase: Negative
Bacitracin susceptibility: Resistant
Novobiocin susceptibility: Resistant

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