CSMLS Complete Solutions

CSMLS Complete Solutions 1° ID of CML results ️BCR/ABL1 positive, 5% blasts A pediatric urine sample with a volume of 3 mL is submitted for microscopic examination. How should the sample be processed? ️examine without spinning Acetone spill procedures ️- Evacuate - Isolate hazard area - Keep out unnecessary and unprotected personnel - Eliminate all ignition sources - Increase ventilation to area or move leaking container to a well-ventilated and secure area After addition of ammonium hydroxide and ammonium oxalate during a decalcification chemical endpoint test there is no visible precipitate. What does this indicate? ️Decalcification is complete An organism grew on MacConkey agar incubated in O2, but failed to grow on blood agar incubated in CO2. What is the most probable cause of this finding? ️Organism likes O2. Autoclave conditions ️103.4 kPa, 121°C, 30-60 minutes CSF collection volumes ️- Chem: 3-5 mL (min. 0.2 mL/test) - Micro: 3-5 mL - Hematology: 3-5 mL (min. 1 mL) - Others (cytology, virology): 3-5 mL (min. 1 mL) Does spinning a clotted specimen affect K levels? ️no Express consent ️-indicates that documents have been signed by the patient or relative-often done when the patient is being admitted to hospital or prior to the procedure itself Failed Δ check flag on a urea result (using the urease enzymatic method) ️NaF anticoagulant used Falsely ↑ U24CREA? ️Include the first morning voided urine in the sample collection HbC Disease Hb levels ️HbC: 90% HbF: 7% (↑) HbA2: 2% (N) HbA: 0% HbC Trait Hb levels ️HbA: 60% HbC: 30% HbA2 & HbF: N Hemolytic disease UMA results ️(-): bilirubin +++: urobilinogen Hepatic jaundice ️↑: CBIL, unconjugated bilirubin ↓: urobilinogen Hepatobiliary obstruction UMA results ️N: urobilinogen +++: bilirubin if erythromycin R and clindamycin S: ️perform D test Key factors in determinant of health: ️- Biology/genetics - Health services - Early childhood development - Income/social status - Employment & working conditions - Education - Support - Social environments - Personal health practices - Physical environment - Gender - Culture Kirby Bauer procedures ️- 0.5 McFarland turbidity std. TSB - inoculate 0.8x10^8 CFU/mL - Agar 4mm thick - Inoculate within 15 min? Liver disease UMA results ️(+)/(-): bilirubin ++: urobilinogen Minimum Inhibitory Concentration (MIC) ️Diffusion method. The lowest [antibiotic] that will inhibit (bacteriostatic/bactericidal) visible growth of a micro-organism. Test done w/ broth dilutions and a control; the last clear tube's antibiotic conc. is the MIC. Negligence example ️Failing to report a critical value Normal crystals in ā urine ️triple phosphate (AMP), calcium oxalate, uric ā/phosphates Normal crystals in alkaline urine ️ammonium biurate (thorny apples), calcium phosphates, calcium carbonates Novobiocin QC organisms ️+: S. saprophyticus -: S. aureus Pale cytoplasmic staining w/ H&E or lacks three distinct shades of pink ️Acidify eosin w/ acetic ā or check pH Pathogenic crystals in UMAMI ️cystine, bilirubin, tyrosine, cholesterol, leucine Post-hepatic jaundice ️N: unconjugated bilirubin ↑: CBIL, cholesterol ↓: urobilinogen (none in urine) Prehepatic jaundice ️N: CBIL ↑: hemolysis, unconjugated bilirubin, urobilinogen Reasons for ↑ phos? ️- 1° hypoparathyroidism - ↓ Mg - ↑ Vit. D Reasons for ↓ phos? ️- 1° hyperparathyroidism - ↓ Vit. D - ↑ insulin Reasons for microchatter seen on a hematoxylin and eosin section? ️- Over-deh