MB ASCP CONNECT EXAM LATEST 2023 EXAM 100 QUESTIONS AND CORRECT ANSWERS WITH RATIONALES(100% VERIFIED ANSWERS)|ALREADY GRADED A

MB ASCP CONNECT EXAM LATEST 2023 EXAM 100 QUESTIONS AND CORRECT ANSWERS WITH RATIONALES(100% VERIFIED ANSWERS)|ALREADY GRADED A+ 1. Which PCR method has the highest specificity? - ANSWER- Nested PCR 2. After an amplification procedure followed by gel electrophoresis, you took a photo of your gel. You notice consistent bands in all your gel lanes. However, you also see what appears to be a faint but noticeable band in your reagent blank lane. What is your best explanation for the reagent blank band? - ANSWER- There was obvious contamination in this PCR reaction 3. A technologist uses the spectrophotometer to quantify the amount of DNA extracted from a blood specimen diluted 1:30. The absorbance reading at 260 nm was found to be 2.545. The concentration of DNA in this extract is: - ANSWER- 3817.5 micrograms/mL 4. This translocation creates a fusion protein between the Abl1 gene on one chromosome and the BCR gene on the other chromosome, resulting in chronic myelogenous leukemia (CML): - ANSWER- t(9;22) 5. The sugar in DNA is: - ANSWER- Deoxyribose 6. Primer dimers are due to what complimentary issue? - ANSWER- 3' to 3' complementarity of primer pairs 7. What is the sequence recognized by poly (A) polymerase? - ANSWER- AAUAAA 8. In what part of a qPCR Amplification Plot do you take your measurement? - ANSWER- Exponential phase 9. In CML, what fusion protein is created? - ANSWER- BCR-Abl1 10. What assay amplifies the probe signal and the probe RNA concentration increases if the target to be detected is present? - ANSWER- Qβ replicase 11. Purines and pyrimidines differ from each other in