ASCP Microbiology Review Questions and Answers

Which of the following is the correct temperature at which an autoclave must be run? -37*C -121*C -25*C 121*C How long is an autoclave run? 15 mins How many lbs per square inch of pressure is necessary when using an autoclave? 15 lbs/sq in Which of the following is the correct depth and pH of a Mueller Hinton plate? -6mm and pH 7.2-7.4 -3mm and pH 6.5-6.8 -4mm and 7.2-7.4 4mm and pH 7.2-7.4 What is the McFarland standard for use in the Kirby Bauer method? 0.5 (10^8 organisms) Which combination of organisms are used as QC for susceptibility testing using the Kirby Bauer method? -S. pyogenes, P. mirabilis, N. lactamica -P. vulgaris, S.pyogenes -E. coli, S.aureus, P. aeruginosa E. coli, S.aureus, P. aeruginosa Which anaerobic bacteria is isolated on Bacteroides Bile Esculin Agar (BBE)? What color do the colonies appear as? -B. fragilis, black colonies -C. difficile, green colonies -B. pertussis, yellow colonies B. fragilis, black colonies Kanamycin-Vancomycin Laked Blood is used to isolated which anaerobic bacteria? -Clostridium species -Legionella species -Bacteroides species Bacteroides species Cycloserine-Cefoxitin Fructose Agar (CCFA) is used to isolate which anaerobic bacteria? -Mycobacteria species -C. diptheriae -C. difficile C. difficile What factors is CDC Anaerobic Blood Agar enriched with?