ASCP CG Exam (Classic Cytogenetics) Exam Prep Questions and Answers

What are the basics of in situ hybridization? Pretreatment of slides with acid, extraction of DNA with barium hydroxide, and incubation in 2xSSC to promote denaturation. The following are indicators for amniocentesis except: A family history of an autosomal recessive disorder. When a bone marrow sample cannot be delivered to the lab within 4 hrs, the sample should: Be put in media and kept at room temp. A PB sample is clotted. What do you do? Break up the clot. All of the following information is required during the specimen set up procedure except: Infectious agent. When should samples be logged in? As soon as they arrive in the laboratory. How long do you wait before requesting an amniotic fluid if no cell attachment is observed? 10 days. All of the following will result in culture failure except: Transport at room temp. Which cells would you want to use for long term serial cytogenetic studies? Fibroblasts. The following enzymes can be used to dissociate chorionic villus samples except for: RNAse. Which of the following is not a necessary ingredient which is added to modified medium? Antibiotics. All of the following have buffering capability except: L-Glutamine. What type of media is necessary for the culture of Fragile X cases? Folic acid deficient media