VDistinction Level BTEC Applied Science Assignment C, Chromatography

VDistinction Level BTEC Applied Science Assignment C, Chromatography. Soluble :solubility is the degree to which a substance dissolves in a solvent to make a solution Solvent : the substance in which a solute dissolved to produce a homogeneous mixture Mobile phase : the liquid that transports the substance mixture through the absorbing material which travels along the stationary phase and carries the substance components with it Stationary phase : the solid material that absorbs the mixture flowing through it Adsorption : the process by which atoms, molecule or ions from a gas or liquid adhere to a surface the process is not permitted Chromatogram : the resulting paper or plate produced showing the substance Immiscible: liquids that do not mix together Porosity : a measure of the volume of tiny holes in a material divided by the total volume of the material measuring rf values When the solvent front reaches the top of the TLC plate or chromatography paper, it is measured to determine how far it has travelled. We can tell how far the solution has moved and how far the spots have migrated by calculating the rf. Draw a line from the solvent front to the original line and measure it with a ruler to see how far the solvent has travelled. Then draw a line from the middle of the spot to the original line and measure it again with a ruler. This would display how far the spot has travelled. This equation may be used to calculate the rf value. rf= distance travelled by substance/ distance travelled by solvent font