Blood banking exam 1
A one step test that detects in vivo coating of an individuals red blood cells with
IgG or complement - CORRECT ANSWER-Direct Antiglobulin Test (DAT)
When are positive DATs seen? - CORRECT ANSWER-Acute and delayed
hemolytic transfusion reactions, autoantibodies, and drug induced hemolytic
events
After getting a positive DAT how can it be evaluated further? - CORRECT
ANSWER-Elution and adsorption
Removing an antibody that is attached to the surface of a red blood cell (acidic
glycine may be used) - CORRECT ANSWER-Elution
Procedure to bind antibodies to red blood cell antigens in order to remove
antibodies from plasma and analyze antibodies that may remain behind -
CORRECT ANSWER-Adsorption
An antibody formed in response to pregnancy or transfusion targeted against an
antigen that is not present on persons red blood cell - CORRECT
ANSWER-Alloantibody
What is the anti-A1 lectin? - CORRECT ANSWER-Dolichos biflorus
What is the anti-H lectin? - CORRECT ANSWER-Ulex europeaus
An antibody that is targeted against antigens that are present on the patient own
red cells - CORRECT ANSWER-Autoantibody
Check cells are red blood cells coated with what? - CORRECT ANSWER-IgG
Done to check for the presence of antibody and/or complement coating red cells
following incubation with serum or plasma - CORRECT ANSWER-Indirect anti
globulin test
, The IAT is ______ testing and requires a ______phase - CORRECT ANSWER-In
vitro
Incubation phase
DCe - CORRECT ANSWER-R1
DcE - CORRECT ANSWER-R2
DCE - CORRECT ANSWER-Rz
Dce - CORRECT ANSWER-R
dCe - CORRECT ANSWER-r'
dcE - CORRECT ANSWER-r2
dCE - CORRECT ANSWER-ry
dce - CORRECT ANSWER-r
Gene that makes ABO precursor on the red cells - CORRECT ANSWER-FUT1
Gene that makes precursor that makes soluble in body fluids - CORRECT
ANSWER-FUT 2
Gene encodes lewis antigens - CORRECT ANSWER-FUT3
What is the name of the precursor substance from which A and B antigens are
made? - CORRECT ANSWER-H
What is the terminal sugar on this precursor? - CORRECT ANSWER-Fucose
What sugar must be added to the precursor for A antigens to be expressed? -
CORRECT ANSWER-N- acetylgalactosamine
What sugar must be added to the precursor for B antigens to be expressed? -
CORRECT ANSWER-galactose
A one step test that detects in vivo coating of an individuals red blood cells with
IgG or complement - CORRECT ANSWER-Direct Antiglobulin Test (DAT)
When are positive DATs seen? - CORRECT ANSWER-Acute and delayed
hemolytic transfusion reactions, autoantibodies, and drug induced hemolytic
events
After getting a positive DAT how can it be evaluated further? - CORRECT
ANSWER-Elution and adsorption
Removing an antibody that is attached to the surface of a red blood cell (acidic
glycine may be used) - CORRECT ANSWER-Elution
Procedure to bind antibodies to red blood cell antigens in order to remove
antibodies from plasma and analyze antibodies that may remain behind -
CORRECT ANSWER-Adsorption
An antibody formed in response to pregnancy or transfusion targeted against an
antigen that is not present on persons red blood cell - CORRECT
ANSWER-Alloantibody
What is the anti-A1 lectin? - CORRECT ANSWER-Dolichos biflorus
What is the anti-H lectin? - CORRECT ANSWER-Ulex europeaus
An antibody that is targeted against antigens that are present on the patient own
red cells - CORRECT ANSWER-Autoantibody
Check cells are red blood cells coated with what? - CORRECT ANSWER-IgG
Done to check for the presence of antibody and/or complement coating red cells
following incubation with serum or plasma - CORRECT ANSWER-Indirect anti
globulin test
, The IAT is ______ testing and requires a ______phase - CORRECT ANSWER-In
vitro
Incubation phase
DCe - CORRECT ANSWER-R1
DcE - CORRECT ANSWER-R2
DCE - CORRECT ANSWER-Rz
Dce - CORRECT ANSWER-R
dCe - CORRECT ANSWER-r'
dcE - CORRECT ANSWER-r2
dCE - CORRECT ANSWER-ry
dce - CORRECT ANSWER-r
Gene that makes ABO precursor on the red cells - CORRECT ANSWER-FUT1
Gene that makes precursor that makes soluble in body fluids - CORRECT
ANSWER-FUT 2
Gene encodes lewis antigens - CORRECT ANSWER-FUT3
What is the name of the precursor substance from which A and B antigens are
made? - CORRECT ANSWER-H
What is the terminal sugar on this precursor? - CORRECT ANSWER-Fucose
What sugar must be added to the precursor for A antigens to be expressed? -
CORRECT ANSWER-N- acetylgalactosamine
What sugar must be added to the precursor for B antigens to be expressed? -
CORRECT ANSWER-galactose
