ACE ASCP Final Exam Questions and Answers, Latest Updated 2024/2025 | Rated A+

Which of the following condition is the most common cause of increase anion gap? A.Metabolic alkalosis B. Metabolic acidosis Respiratory acidosis Respiratory alkalosis B. Metabolic acidosis High anion gap metabolic acidosis is caused generally by the body producing too much acid or not producing enough bicarbonate. This is often due to an increase in lactic acid or ketoacids, or it may be a sign of kidney failure. More rarely, high anion gap metabolic acidosis may be caused by ingesting methanol or overdosing on aspirin. Which of the following enzymes has the longest elevation after Myocardial Infarction (MI)? CK-MB Myoglobin Troponin AOTA C. Troponin ACE ASCP Final Exam Questions and Answers, Latest Updated 2024/2025 | Rated A+ Troponin is elevated up to 10-14 days after Myocardial Infarction. Lactic acid specimen: Chilled and separated from cells Heated Room temperature Request EDTA sample only A. Chilled and separated from cells Feedback Special Handling: No tourniquet. Place on ice immediately and deliver to lab or aliquot within 15 minutes. DO NOT FREEZE WHOLE BLOOD SPECIMEN. Specific gravity measured by refractometer: 1.035. Protein and glucose both trace in reagent strip: Presence X-ray medium Albuminuria Glucosuria Normal A. Presence X-Ray medium Feedback Xray medium/Radiographic dye can cause erroneous results in urine specific gravity measurement. A patient with walking pneumonia is being treated with penicillin. State one possible condition why the patient is not recovering: The organism produces beta lactamase The organism has no cell wall The organism has capsule The organism produces an enzyme that neutralizes the antibiotic B. The organism has no cell wall. Feedback Pneumonia caused by Mycoplasma pneumoniae is routinely treated with antibiotics, although the disease is usually self-limiting. The disease is treated with macrolide, tetracycline, or fluoroquinolone classes of antibiotics. All mycoplasmas lack a cell wall and, therefore, all are inherently resistant to beta-lactam antibiotics (e.g., penicillin). What is the coefficient of variation if the mean is 90 and the standard deviation is 5.48? 2.75 6.09 5.68 30 B. 6.09 Feedback: CV= SD/meanX100, (5.48/90)X100= 6.09. Blood smear below denotes Hairy Cell Leukemia (HCL), which of the following test is used to identify this type of leukemia? Image. 1 Myeloperoxidase Tartrate-resistant acid phosphatase Tartrate-resistant alkaline phosphatase Sudan Black B. Tartrate-resistant acid phosphatase Feedback: Hairy cells demonstrate strong positivity for tartrate-resistant acid phosphatase (TRAP) staining. The hairy cells are larger than normal and positive for CD19, CD20, CD22, CD11c, CD25, CD103, and FMC7. Use to differentiate Salmonella from Citrobacter: Lysine decarboxylase ONPG Indole Vogues Proskauerterm-9 A. Lysine decarboxylase Feedback: Salmonella (+); Citrobacter (-) Arrange the common Rh antigens according to immunogenicity (greatest to least): DcECe DCEce DcEeC CDcEe A. DcECe Feedback Most immunogenic D c E C e least immunogenic. Most common error in Polymerase Chain Reaction (PCR) is NUCLEIC ACID CONTAMINATION. A True (Correct Answer) B False A. True What disease is associated with the possession of HLA-B27? Ankylosing spondylitis Hashimoto's Disease Goodpasture's syndrome SLE A. Ankylosing spondylitis Feedback: Ankylosing spondylitis belongs to a group of arthritis conditions that tend to cause chronic inflammation of the spine (spondyloarthropathies). The HLA-B27 gene can be detected in the blood of most patients with ankylosing spondylitis. Burr cells are seen in the peripheral blood smear, this is indicative of? (Fig. 2) Uremia (Kidney Disease) Anemia Leukemia Liver Disease A. Uremia (kidney Disease) Feedback: The above shown picture illustrates Burr cells, these cells are indicative of Uremia (kidney disease) Anti IgG (-); C3d (+): What should the MLS do? Elution IAT DAT Pre-warm D. Pre-warm Feedback Prewarm technique can be used to prevent cold-reactive alloantibodies or autoantibodies from reacting in the IAT phase. Specifically, prewarm technique prevents cold antibodies from binding complement at RT (as opposed to 37o C ) and subsequently being detected by anti-C3 in the IAT by polyspecific AHG serum. Maximum interval which a recipient sample maybe used for crossmatching if the patient has been recently transfused, has been pregnant? 2 days 6 days 3 days NOTA C. 3 days CIN (Cefsulodin, Irgasan, Novobiocin) Agar is a recommended culture medium for the recovery of: Rhodococcus equi Cardiobacterium hominis Brucella suis Yersinia enterocolitica D. Yersinia enterocolitica Feedback: CIN Agar is recommended for use in the selective and differential isolation of Yersinia and Aeromonas species from clinical specimens, environmental samples, and food sources. Yersinia enterocolitica will exhibit bull's eye appearance on the said agar. What is the formula for sensitivity? TP/(TP+FN)x100 TP/(TP+FP)x100 EMAIL ME: For help with report, Assignment, Essay and thesis writing. (TP+FN)/TPx100 (TP+FP)/TNx100 A. TP/(TP+FN)x100 CThe anemias of the qualitative hemoglobinopathies, such as sickle cell anemia, are morphologically classified as: Microcytic, hypochromic Macrocytic, normochromic Normocytic, normochromic NOTA C. Normocytic, normochromic According to AABB standards, Fresh Frozen Plasma (FFP) must be infused within what period of time following thawing? 24 hrs 36 hrs 48 hrs 72 hrs A. 24 hours A woman having menstrual cycle would have the ff. result: Increased TIBC Increased Ferritin Increase % Saturation Normal Transferrin A. Increased TIBC Feedback A total iron binding capacity value above 450 mcg/dL usually means that there's a low level of iron in your blood. This may be caused by a lack of iron in the diet, increased blood loss during menstruation, pregnancy, or a chronic infection. Causative agent of "Cat bite fever" Pasteurella multocida Actinomyces israelii Bartonella henselae Leptospira interrogans A. Pasteurella multocida Feedback Pasteurella species are commonly isolated pathogens in most animal bites, especially in dog- and cat-related injuries. These injuries can be aggressive, with skin manifestations typically appearing within 24 hours following a bite. These wounds can exhibit a rapidly progressive soft-tissue inflammation that may resemble group A β-hemolytic Streptococcus pyogenes infections. What is the sugar fermented by Staphylococcus aureus? Mannitol Sucrose Glucose Maltose A. Mannitol Mannitol salt agar is a commonly used growth medium in microbiology. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. It contains a high concentration (7.5%-10%) of salt (NaCl), making it selective for gram positive bacteria Staphylococci (and Micrococcaceae) since this level of NaCl is inhibitory to most other bacteria. It is also a differential medium for mannitol fermentors, containing mannitol and the indicator phenol red. Staphylococcus aureus produce yellow colonies with yellow zones, whereas other Staphylococci produce small pink or red colonies with no colour change to the medium. If an organism can ferment mannitol, an acidic byproduct is formed that will cause the phenol red in the agar to turn yellow. Identify the organism: Fig. 3 Blastomyces dermatitidis Malasezia furfur Alternaria Candida albicans A. Blastomyces dermatitidis Feedback The above image show broad base budding. What is the first step in agglutination? Sensitization Precipitation Diffusion Lattice formation A. Sensitization Feedback Agglutination involves two process: First, sensitization or initial binding. Second, lattice formation or formation of large aggregates. Sterilization of autoclave: 121°C at 20 lbs/psi for 30 minutes 121°C at 15 lbs/psi for 15 minutes 85°C for 15 lbs/psi 30 minutes 100°C for 20 lbs/psi 50 minutes B. 121°C at 15 lbs/psi for 15 minutes