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BSC 2010L UPDATED Exam Questions and CORRECT Answers

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What does a spectrophotometer measure?measures the amount of light that is absorbed by (on a scale of 0-2) or transmitted through (on a scale of 0-100) a sample, at particular wavelengths of light How is a spectrophotometer measured?The spectrophotometer is used to discover at what wavelength bromophenol blue absorbs the most light; the optimum wavelength for measuring light absorption by the dye What is an absorption spectrum?the pattern of light absorption over a range of wavelengths for a particular substance; used to determine the wavelength of peak absorbance by the substance What value for a substance can be determined by plotting its absorption spectrum?an unknown concentration of a sample; Can determine the wavelength of maximum absorbance by the substance.

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