Microbiology Semmelweis Questions with 100% Correct Answers | Latest Version 2024 | Verified

What does sterilization mean? - Killing procedure of any kind of germs What does disinfection mean? - Procedure where the number of the germs are reduced to a safety level. Which parameters can influence the effectivity of the sterilization? - The number of the germs, the resistance of the germs, the concentration of the disinfectants, the presence of the organic materials, the initial time, the presence of the biofilm. Parameters of the hot-air sterilization cupboard protocol? - 180°C; 1 hour, 160°C; 2 hours, 140°C; 3 hours. Parameters of the autoclaving? - + 1 atm overpressure, 121°C, 20-30 minutes or 134°C, +2 atm overpressure 10 minutes. Chemical agents used for gas sterilisation? - Etilene oxide, formaldehyde, beta-propiolacton The theoretical background of plasma sterilisation? - Hydrogen-peroxide in high electric field will form plasma stage. The produced free radicals will kill the microbes. At the end of the procedure will be produced water, oxygen and other nontoxic products. Biological method used for checking the effectivity of the sterilisation. - By Bacillus/Geobacillus stearothermophilus spores. If the procedure was performed in correct way, the spores cannot be cultivated.