CSMLS chemistry exam questions and answers

CSMLS chemistry exam questions and answers Chilled Samples - CORRECT ANS-ACTH, lactic acid, ammonia, blood gases, acetone, gastrin, glucagon, pyruvate, renin Protect samples from light - CORRECT ANS-bili, carotene, erythrocyte protoporphyrin, vitamin A, vitamin B12 Warm Samples - CORRECT ANS-Cold agglutinins, cryoglobulins Fasting samples - CORRECT ANS-Fasting blood sugar, triglycerides, lipid panel, gastrin, insulin Formalin pigment - CORRECT ANS-Aka acid formalin hematin. Brown, granrular, doubly refractile deposit Remove with picric acid after 100% alcohol in rehydration step. Tissue will be yellow. Remove yellow discolouration with RTW (30min), acid alcohol + wash in RTW, prolonged alcohol wash and lithium carbonate in 70% alcohol Mercury Pigment - CORRECT ANS-Dark brown to black crystals. Remove during dehydration: 20mL of strong alcoholic iodine From sections: bring to water, place in 0.5% alcoholic iodine or gram's iodine, rinse with water, place into sodium thiosulphate until brown discolouration gone. Hematoxylin - CORRECT ANS-Not a true dye. Must be oxidized to hematein (ripened by exposure to atmospheric oxygen, sodium iodate, mercuric oxide, potassium permanganate) Hematein - CORRECT ANS-Weak, anionic dye. Oxidation product of hematoxylin, active coloring agent Little affinity for tissue, but becomes strong dye when combined with metallic mordant (alum salts, iron salts)