BMSC 210 Midterm 1 Review | 100% Correct Answers | Verified | Latest 2024 Version

Describe Koch's postulates and the significance of his experiment - Koch's postulates: with the use of anthrax provided a basis for Germ Theory. the experiment: extract blood from diseased individual grow culture put culture into healthy individual, develop disease extract this blood, culture it to show same results Describe the importance of Louis Pasteur and the significance of his swan-neck flask experiment - Disproved spontaneous generation (miasma) the swan-neck made contamination- and growth- visible Why are viruses not considered living? - they do not perform metabolism and they are parasitic (cannot grow on own), also missing cytoplasm How do light microscopes work? What light microscopes did we learn about? - Use of light to make observation, can see live specimen Brightfield: absorb and scatter light, use gram staining (due to lack of pigment), can observe motility Darkfield: specimen deflect and scatter light Phase-Contrast: very detailed, high contrast Differential Interference Contrast: #D w/ use of 2 light beams Fluorescence: use of fluorochromes, UV light, and excitation (confocal: 3D fluorescence) How do electron microscopes work? What types have we learned about? - Use of electrons and magnets, higher mag/res, but specimen must be dead TEM: go through specimen, can see inside SEM: electrons bounce off