D103 Review study Guide Questions with Complete Solution 2024/2025 Update
D103 Review study Guide Questions with Complete Solution 2024/2025 Update Principles of immunofluorescence staining we want to identify 2 dif proteins, how do we do it? *Make sure that ........ (2 essential parts to note)First we must generate primary antibodies that recognize/bind our target protein from 2 DIFFERENT species animals. then, use secondary antibody (tagged with color) from a 3rd animal that recognizes/binds the primary but is a dif color than the other secondary antibody *primary antibodies must be from different species *secondary antibodies must be a dif. color than each other, must be dif animal than primary animal, but can be from same animal as other secondary so basically, at least 3 dif animals required, refer to slide for better understanding rabbit ;;;;;;;;;;;;;;;;;;;;;;;;;;;;mouse goat ;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;; goat how and what is Tagging used for?through protein fusion of a fluorescent protein, we can "tag" a protein of interest/target, making it fluorescent, so that we can see/monitor behavior GFPgreen fluorescent protein, used for tagging, when shine blue light on it, reflects & fluoresces green light back
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