ASCP Practice Questions - Histotechnology verified to pass 2024

ASCP Practice Questions - Acetic acid - correct answers This fixative does not fix or destroy carbohydrates, and it does not fix lipids. It penetrates very rapidly and leaves tissue very soft. It is added to many fixative mixtures because of its ability to fix nuclei. Glutaraldehyde - correct answers Techniques using Schiff reagent, such as the periodic acid-Schiff (PAS) stain, cannot be used on __________ fixed tissues because false positive results will be obtained. Glyoxal - correct answers This fixative is the smallest dialdehyde and is used in the textile industry for imparting wrinkle resistance and permanent press creases. It has replaced formaldehyde in some histopathology laboratories because it is much less toxic. Zinc salts - correct answers Today, _________ has/have found use not only in combination with formaldehyde for routine fixation, but as a substitute for mercury in the B-5 solution commonly used for the fixation of lymph node and bone marrow tissues Michel transport medium - correct answers If unfixed tissue is to be held for several days or transported over a long distance then this transport medium is recommended. Dehydration, clearing, infiltration - correct answers Tissue processing is usually considered to include: Xylene - correct answers The most widely used clearing agent in histology today is: Ethylenediaminetetraacetic acid (EDTA) - correct answers This chelating agent binds calcium ions and is one method used for decalcifying tissues. Phase-contrast microscope - correct answers This microscope is used for the examination of unstained specimens, especially unstained living cells, and allows almost transparent objects to be seen clearly.