ImmunoSero Lab - Q1 [Laboratory Safety]

ImmunoSero Lab - Q1 [Laboratory Safety] In general, we need to know the means to protect oneself, coworkers and civilians (patients) from different types of hazards present in the laboratory. - ANSWER-WHY STUDY LABORATORY SAFETY AND WASTE DISPOSAL? Personal Protective Equipment Hand washing Labelling Decontamination - ANSWER-Precautions Physical Biological Sharp Electrical Fire and Explosive Radioactive Chemical Ergonomic Cryogenic - ANSWER-Types of Hazards Chemical waste Radioactive waste Biohazardous waste - ANSWER-Waste Disposal Treat ALL human blood samples and other body fluids as infectious (containing bloodborne pathogens such as HIV, HBV, etc.) - ANSWER-The Universal Precaution (OSHA) Prevention of all kinds of infection spread via: • Hand hygiene • Use of PPE • Respiratory hygiene and cough etiquette • Safe injection practices • Safe handling of potentially contaminated surfaces in the patient environment. - ANSWER-The Standard Precaution (CDC) Chemical Fume Hood - ANSWER-Expels noxious and hazardous fumes from chemical reagents. Biosafety Cabinet - ANSWER-- Removes potentially harmful particles to the person working with biologic samples. - Has four described levels of biosafety CDC) The Chain of Infection - ANSWER-A continuous link between a source, a method of transmission and a host. 1. The source contains potentially harmful organisms 2. Microorganisms are transmitted to the host 3. The infected host becomes another source for disease transmission - ANSWER-The Chain of Infection Personal Protective Equipment (PPE) - ANSWER-Protects the user against health or safety risks at work. Protects the mucosal surfaces of the face and mouth. Prevents inhalation of airborne pathogens. 1. Laboratory Gown ⇒ Must be full sleeved, buttoned and made with water resistant material. 2. Safety Goggles ⇒ Covers both eyes to protect from splashes. 3. Face Shields ⇒ (Optional) Full-face protection from splashes. 4. Gloves ⇒ Can be made from Latex or Polyvinyl. Must be changed for every patient. 5. Masks ⇒ Surgical masks are acceptable, but masks such as N95 must be worn upon handling patients with Mycobacterium tuberculosis infection. - ANSWER-SAMPLE OF PPE'S IN THE LABORATORY Hand Washing - ANSWER-Must be done frequently and to break the chain of