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BIOD 171/Microbiology Lab 8 Exam Portage Learning (Already Graded A)

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BIOD 171/Microbiology Lab 8 Exam Portage Learning (Already Graded A) AttemptHistory Attempt Time Score LATEST Attempt 1 42 minutes 22.25 out of 30 Scoreforthisquiz:22.25outof30 SubmittedJun2at8:44pm Thisattempttook42minutes. Theword‘antigen’isactualyacombinationforwhattwowords? YourAnswer: eanswhenantigen entersthebodyproducesofantibodieswilstart Antibody-generating FortheELISAassaydepictedbelow,whichstep(A-E)firstcontainsonlyspecificaly boundantigens(akaa'pure'population)? YourAnswer: C SpecificalyboundantigensarepresentinstepC.Specificandnon-specific antigensarepresentinBbutthenthewashstepremovesthenon-specific (unbound)antigens,leavingonlythespecificpopulationseeninstepC. Whatwouldhappenintermsofthecolorimetricreadoutifyouforgottodothewashstep afterStepD(above)? Question4 2.5/2.5pts Question5 2.5/2.5pts Question6 1.25/2.5pts Question7 0/2.5pts YourAnswer: theunboundsecondaryantibodywilnotbewashedaway,sowemayhavefalse positiveresult Alofthewelswouldhaveadarkcolor.Theintensityofthereadoutisdependent upontheamountofthelabeledsecondaryantibodypresentinthewel.Sincethe unboundsecondaryantibodywasnotwashedaway,alwelswouldreactequaly. Awesternblotseparatesproteinsbasedon . YourAnswer: Sizeandmolecularmas

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