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Exam (elaborations)

ACS BIOCHEMISTRY EXAM UPDATE 2023&2024 GRADED A+ 100% ACCURATE

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ACS BIOCHEMISTRY EXAM UPDATE 2023&2024 GRADED A+ 100% ACCURATE Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - ANSWER Separates sample based on size with smaller molecules eluting later. Ion-Exchange Chromatography - CORRECT ANSWER Separates sample based on charge. CM attracts +, DEAE attracts -. May have repulsion effect on like charges. Salt or acid used to remove stuck proteins

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  • acs biochemistry exam update graded a
  • henderson hasselbach equation answer ph pka
  • fmoc chemical synthesis answer used in synthesis
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