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BIOD 171 PL04 Intoduction to Growth Media: Lab 4 notebook (Portage learning) FALL 2023
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Title: PL04 Growth Media Objective: To understand the types and uses of growth media for the isolation and identification of unknown bacterial samples. Procedure: 4-Phase Dilution Streaking: Clonal isolation 1. Using sterile loop spread culture in area#1. 2. Using a new sterile loop drag through the end of area#1 once. 3. Using a new sterile loop drag through the end of area#2 once. 4. Using a new sterile loop drag through the end of area#3 once. • Use a back-and-forth pattern to dilute the culture in each zone. • Invert plate and incubate overnight at 37ºC. Using LB agar plate (it’s non-selective so we will get something growing). [Non-selective agar] Quadrant Growth: Rapid test for multiple isolates
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BIOD 171
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BIOD 171
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- Institution
- BIOD 171
- Course
- BIOD 171
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- Uploaded on
- November 27, 2023
- Number of pages
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- Written in
- 2023/2024
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- Other
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biod 171 pl04 intoduction to growth media lab 4 n
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