BioChem C785 study guide PA

BioChem C785 study guide PA Complementary DNA and/or RNA molecules pair with each other by hydrogen bonding during replication, transcription, or translation. Which of the following pairs of molecules are complementary? (Select all that apply) Correct Answer: tRNA and mRNA mRNA and template DNA During DNA replication the parent strands ______ and DNA polymerase______. Correct Answer: separate, creates the new DNA strands The following sequence is one strand of the DNA that makes up the SNCA gene that codes for alphasynuclein, a protein found in the brain. 5' ATGCGGTGACGG 3' Which of the following is the complementary second DNA strand? Correct Answer: 5' CCGTCACCGCAT 3' Assume you start with one copy of target DNA. How many copies of DNA will be made after 4 rounds of PCR (assume 100% reaction efficiency)? Correct Answer: 16 What are the necessary ingredients for a PCR reaction? Correct Answer: dNTPs; thermostable DNA polymerase; DNA primers; double-stranded DNA A frameshift mutation changed this sequence 5' AAC GCT TAG 3' to which of the following? Correct Answer: 3' GAT TCG CA 5' Which choice represents a silent mutation for this sequence: 5' GGU UGC ACU 3'? Correct Answer: 5' GGU UGC ACA 3' Beta thalassemia is a disease that causes low levels of hemoglobin. To inherit this autosomal disease, an individual must receive two mutant alleles. Which of the following likely represents the chromosome pairs of a healthy parent who has a child with beta thalassemia? (The yellow band represents the mutant allele and the green band represents the normal allele.) * Correct Answer: Option 3- The chromosomes of a healthy parent who has a child with beta thalassemia must be a carrier of the disease, i.e. the parent must have one copy of the (dominant) normal allele and one copy of the (recessive) mutant allele. Your black dog and your friend's white dog have puppies. If inheritance of coat color is co-dominant, the coat color of the puppies will be ________________, the color will be _______________ if incompletely dominant, and it will be _______________ if it is complete dominant Correct Answer: Black-and-white spotted, gray, black Freidreich's ataxia is an autosomal recessive disorder that results from multiple GAA insertions into the FXN gene. Which of the following best illustrates the inheritance pattern of Freidreich's ataxia in a family with the disease? Correct Answer: None of these pedigrees are consistent with an autosomal recessive pattern. The pedigree chart for Option 3 shows evidence of carriers, and both males and females have Friedrich's ataxia in this chart. These properties of the chart indicate that it is for an autosomal recessive disease.