Biology Lab Exam 2 study questions & answers

Biology Lab Exam 2 study questions & answers Which enzyme is responsible for reading the DNA and creating a new copy? - ANSWER-DNA Polymerase Complementary base pairs in the DNA double helix are held together by three type of week bonds. Which is NOT the chemical bond that hold to strands together? - ANSWER-Covalent Bonds Consider a circular DNA plasmid that has been cleaved (cut) once a by a restriction enzyme. Which type of cleavage would be the most difficult to reconnect? - ANSWER-blunt ends Which enzyme is responsible for breaking the hydrogen bonds that hold DNA together? - ANSWER-Helicase The cloning of a DNA fragment requires connecting (gluing) the gene to a plasmid after both have been digested with the same restriction enzyme. Ligase is the enzyme that connects DNA and plasmid fragments together. - ANSWER-True Which enzyme is responsible for joining segments of DNA? - ANSWER-Ligase Which enzyme is responsible for inserting short, RNA primers on the lagging strand so the DNA can be replicated? - ANSWER-Primase Polymerase reads DNA continuously in the 5' to 3' direction (toward the fork) and can only add nucleotides to the 3' end of a DNA strand . - ANSWER-False During replication, a gap is formed between the two STRANDS of DNA. This is called the _____. - ANSWER-Replication Bubble Adjacent nucleotides in the DNA strand (backbone) are held together by what type of interaction? - ANSWER-Covalent Bonds