ASCP MB Final Exam Practice Questions and Answers (Already Graded A+ Latest Verified 2023)

ASCP MB Final Exam Practice Questions and Answers (Already Graded A+ Latest Verified 2023) What type of restriction enzyme lacks methylation activity? Restriction Enzyme Type II What is necessary to make cDNA? Reverse transcriptase Number of SNPs in a human genome? 11 billion (10^7) How many genes (base pairs) are in a human haploid genome? 3 billion base pairs The polymerization of acrylamide requires: Catalyst (APS or TEMED) or Light activation How does agarose gel pore size relate to concentration? Inversely proportional What is denatured during a southern blot? Hydrogen bonds (inter-strand bonds) Melting temperature formula Tm = (4C x GC Pairs) + (2C x AT pairs) Which is likely to interfere with fluorescent probe? 5' G Proper GC content of primer 35-65% Inhibitors of PCR Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenol Blue Order to add to a PCR reaction: Master mix patient sample positive control negative control Pre Area Positive Pressure Post Area Negative Pressure Which PCR requires two steps for specificity? Nested PCR Which microorganism is detected by branched DNA amplification? DNA or RNA Which technique uses RNA, antibodies, and DNA? Hybrid Capture Assay Use of bisulfite treatment of DNA to determine its pattern of methylation Bisulfite DNA What tests are isothermal? NASBA, TMA, & SDA Which leukemia is associated with FLT3 gene? AML T-cell gene rearrangement is seen in what malignancy? Sezary Syndrome Which translocation causes Acute Premyelocytic Leukemia? t 15;17 Ewing Sarcoma t 11;22 Synovial Sarcoma t X;18 Follicular Lymphoma t 14;18 What genes would be screened in a colorectal panel? MSH2 & MLH1 What gene mutation causes a high risk of breast or ovarian cancer? BRCA1 and BRCA2 What does MSI test for? Mutations in mismatch repair genes (MMR) What disease is related to factor V coagulation gene (F5 1q23)? Thrombossis How many copies are in a full Fragile X mutation? 200-2000+ CGG repeats What disease is known for numerous mutations and large deletions? Muscular Dystrophy (Duchenne) What HPV causes cervical cancer? 16 & 18 You receive a paraffin embedded specimen for HPV typing, which method should you use? PCR Significant change in HIV viral load: 0.5 log10 Lowest viral concentration that can be detected over background or a negative control in the test assay Limit of Detection (LOD) HCV consensus sequence: G-rich Core Gene What responds poorly to ribavarin/interferon treatment? HCV Type 1B What virus affects patients after kidney transplant? CMV or BKV What exons are tested for HLA Class I? Exons 2 & 3 What exons are tested for HLA Class II? Exon 2 beta When sequencing HLD-DR what is targeted? Exon 2 beta What drug is metabolized by CYP2C19? Clopidogrel (Plavix) What drug tolerance is affected by CYP3A5? Carbamazepine What drug is metabolized by CYP2C9? Warfarin What drug is metabolized by CYP2D6? Codeine Mutation in UGT1A1 affectes metabolism of what drug? Irinotecan Antibiotic resistance mechanism in MRSA? mecA PBP2a resistance to methicillin Which has highest vancomycin resistance? vanA Warfarin causes inhibition of activities on which gene? VKORC1 Her2/neu exhibits an overexpression, what would this indicate? More aggressive growth and metastasis of the tumor cells in human breast cancer. Best condition for long term storage of RNA? (6 months) -70C ethanol How long can blood be stored at room temperature for DNA analysis? 24 hours Best method listed to collect bone marrow specimens: EDTA or ACD tubes How should whole blood be sent? Overnight on ice packs Blood should be stored and transported in: EDTA (purple/lavender top tubes) or ACD (yellow top tubes) How long can whole blood be at 4C before isolation? 72 hours How long to keep obsolete document in lab according to CAP checklist? 2 years If you break a phenol bottle, what should you do? Check MSDS then use spill kit When making 20% SDS, should you use a mask or not? Wear a face mask to avoid inhalation of SDS dust Clinical Sensitivity (SNIP) [TP/(TP+FN)] x 100 Clinical Specificity (SPIN) [TN/(TN+FP)] x 100 Where does the signal come from in pyrosequencing? Luciferase conversion of luciferin to oxyluciferin Primers are usually 18-30 bases, which PCR product should use a shorter primer? Previously amplified product