ENZYME FLASHCARDS
WHAT ARE ENZYMES
ENZYMES REFER TO THE THREE DIMENSIONAL STRUCTURES IN
WHICH IS HELD TOGETHER BY THE STRONG H BONDS, VAN DER
WAAL FORCES AND SALT BRIDGES SUCH AS THE DISULPHIDE
BRIDGE
THE ENZYMES REFER TO THE BIOLOGICAL CATALYSTS IN WHICH
WOULD PROVIDE AN ALTERNATE PATHWAY FOR A REACTION TO
OCCUR THAT REQUIRES LOWER EA TO OCCUR
WHAT ARE THE TWO ENZYME MECHANISM THEORIES?
1. LOCK AND KEY HYPOTHESIS
2. INDUCED FIT THEORY
EXPLAIN THE LOCK AND KEY HYPOTHESIS OF THE ENZYME ACTIVITY?
THIS IS ASSUMING THAT THERE WOULD BE COMPLEMENTARITY
BETWEEN THE SHAPE OF THE SUBSTRATE AND THE ENZYME
HOWEVER, THIS METHOD DOES NOT HIGHLIGHT HOW THE ENZYME
WOULD LOWER THE EA OF THE REACTION
EXPLAIN THE INDUCED FIT THEORY HYPOTHESIS OF ENZYME ACTIVITY?
ENZYME FLASHCARDS 1
, IN THE 1950S, KOSHLAND STATED THAT THERE WOULD BE NO
COMPLEMENTARITY BETWEEN TO THE SUBSTRATE AND ENZYME
IN WHICH, THE FUNCTIONAL GROUPS OF THE ENZYMES GO
THROUGH CONFORMATIONAL CHANGES
THROUGH THESE CONFORMATIONAL CHANGES, THE ENZYME
WOULD BECOME MORE SPECIFIC TO THE SUBSTRATE
THROUGH THESE CONFORMATIONAL CHANGES MADE BY THE VAN
DER WALL FORCES, THERE WOULD BE MORE EFFICIENT BINDING TO
THE SUBSTRATE
TIGHTER BINDING ELICITED
WHAT IS THE TRANSITION STATE OF THE ENZYME?
THIS IS CONSIDERED THE EXTREMELY UNSTABLE INTERMEDIATE OF
THE CHEMICAL REACTION
WHAT WOULD THE ENZYME DO TO THE TRANSITION STATE MOLECULE?
THIS IS WHEN THE ENZYME WOULD WRAP AROUND THE
TRANSITIONAL STATE MOLECULE
IN WHICH, IT WOULD LOWER THE ACTIVATION ENERGY
WHAT WOULD OCCUR IF THE ENZYME BINDS TO THE SUBSTRATE IN
TERMS OF FREE ENERGY (DELTA G)
BASED ON THE LOCK AND KEY HYPOTHESIS, WHEN THE ENZYME
BINDS TO THE SUBSTRATE, IT WOULD RESULT IN THE STABILISATION
OF THE SUBSTRATE
IN WHICH, THIS WOULD INCREASE THE DELTA G AND CONSIDERED
COUNTER PRODUCTIVE
ENZYME FLASHCARDS 2
WHAT ARE ENZYMES
ENZYMES REFER TO THE THREE DIMENSIONAL STRUCTURES IN
WHICH IS HELD TOGETHER BY THE STRONG H BONDS, VAN DER
WAAL FORCES AND SALT BRIDGES SUCH AS THE DISULPHIDE
BRIDGE
THE ENZYMES REFER TO THE BIOLOGICAL CATALYSTS IN WHICH
WOULD PROVIDE AN ALTERNATE PATHWAY FOR A REACTION TO
OCCUR THAT REQUIRES LOWER EA TO OCCUR
WHAT ARE THE TWO ENZYME MECHANISM THEORIES?
1. LOCK AND KEY HYPOTHESIS
2. INDUCED FIT THEORY
EXPLAIN THE LOCK AND KEY HYPOTHESIS OF THE ENZYME ACTIVITY?
THIS IS ASSUMING THAT THERE WOULD BE COMPLEMENTARITY
BETWEEN THE SHAPE OF THE SUBSTRATE AND THE ENZYME
HOWEVER, THIS METHOD DOES NOT HIGHLIGHT HOW THE ENZYME
WOULD LOWER THE EA OF THE REACTION
EXPLAIN THE INDUCED FIT THEORY HYPOTHESIS OF ENZYME ACTIVITY?
ENZYME FLASHCARDS 1
, IN THE 1950S, KOSHLAND STATED THAT THERE WOULD BE NO
COMPLEMENTARITY BETWEEN TO THE SUBSTRATE AND ENZYME
IN WHICH, THE FUNCTIONAL GROUPS OF THE ENZYMES GO
THROUGH CONFORMATIONAL CHANGES
THROUGH THESE CONFORMATIONAL CHANGES, THE ENZYME
WOULD BECOME MORE SPECIFIC TO THE SUBSTRATE
THROUGH THESE CONFORMATIONAL CHANGES MADE BY THE VAN
DER WALL FORCES, THERE WOULD BE MORE EFFICIENT BINDING TO
THE SUBSTRATE
TIGHTER BINDING ELICITED
WHAT IS THE TRANSITION STATE OF THE ENZYME?
THIS IS CONSIDERED THE EXTREMELY UNSTABLE INTERMEDIATE OF
THE CHEMICAL REACTION
WHAT WOULD THE ENZYME DO TO THE TRANSITION STATE MOLECULE?
THIS IS WHEN THE ENZYME WOULD WRAP AROUND THE
TRANSITIONAL STATE MOLECULE
IN WHICH, IT WOULD LOWER THE ACTIVATION ENERGY
WHAT WOULD OCCUR IF THE ENZYME BINDS TO THE SUBSTRATE IN
TERMS OF FREE ENERGY (DELTA G)
BASED ON THE LOCK AND KEY HYPOTHESIS, WHEN THE ENZYME
BINDS TO THE SUBSTRATE, IT WOULD RESULT IN THE STABILISATION
OF THE SUBSTRATE
IN WHICH, THIS WOULD INCREASE THE DELTA G AND CONSIDERED
COUNTER PRODUCTIVE
ENZYME FLASHCARDS 2
