LAT Laboratory Animal Technician Questions and Answers Graded A+

LAT Laboratory Animal Technician Questions and Answers Graded A+ U.S. Government provides approximately how much in research/development funds spent by college and university? Answer 2/3 What information is important on grant applications to request funds? Answer Detailed description of research goals, scientific basis of study, and review of relevant literature and all procedures to be performed What is the difference between NIH & NSF? Answer NIH: primary granting agency: academia, federal agencies, biomedical research NSF: Basic research in math, medicine, and biology The USDA stands for what? Answer United States Department of Agriculture The USDA is responsible for what? Answer Enforces and amends AWA Auction, sale, exhibit, transport, breeding, or conduct of research with animals Animals exempt from AWA Research institutions mst register with Supply annual reports Inspection records through the FOIA Unscheduled inspection once per year What does the FOIA stand for? Answer Freedom of Information Act - provides the USDA's inspection records What is the Public Health Service Policy? Answer Institutions which receive funding from PHS and NIH must follow these guidelines, requires filing of an Amimal Welfare Assurance document that demonstrates the institution is following the Guid, PHS policy, AWA, and any other applicable laws The FDA has many responsibilites including: Answer Assures that new drugs and medical devices are safe and effective before approved for use in humans Monitors drug approval Includes human, and veterinary drugs, food, and medical devices Requires that effectiveness be demonstrated through well controlled investigations What is GLP? Answer Good Laboratory Practices Established in 1978 with implementation of laboratory audits and inspection programs Details requirements for QA and SOPs What is the importance of importation and transportations regulations? Answer For the protection of humans and environment from potential disease carrying organisms Which agencies control importation? Answer CDC, USDA, and US Fish & Wildlife Service Institutional polices are established for: Answer facility operations care and use procedures veterinary support physical plant Standard operating procedures relate to: Answer wall aspects of animal care and support personnel management health surveillance of animals and personnel equipment investigators What are the functions of IACUCs and who mandates? Answer mandated by AWA, PHS policy, and the Guide Review and approve protocols for animal use Review animal use program and perform animal facilities inspections at least every 6 months What has to happen before animal research can begin? Answer principal investigator provides written details of how animals will be used in the research project IACUC must review all protocols using animals and must approve all protocols before any animal research can begin What does the IACUC determine? Answer Can deny permission ro start a project can stop a research peoject Ensures compliance with regulations Reviews complete protocols at least every 3 years; reviews updates to protocols yearly What is the AAALAC, International? Answer Association for Assessment and Accreditation of Laboratory Animal Care, International Non-profit organization that provides a mechanism for peer review of animal care and use programs by scientific community Detailed application with information on facilities, procedures, programs, and research animals to use Annual report must be submitted to AAALAC on status of animal care program Site inspection and program evaluation every 3 years Why is it important to keep accurate, up-to-date records? Answer Determine if procedures are followed according to established standards Determine whether environmental controls are properly maintained Improve facility efficiency and effectivness What is PHS Policy? Answer All assurance records related directly to grant applications, research proposals, and changes of research activities 3yrs after completion Centralized Answer Serves all the institution's animal care needs; 1 director for entire institution; All personnel associated with the animal facility; All other purchases, per diem, depreciation, inventories, operational costs, and supplies Decentralized Answer Each department has own animal facility and operating budget; each department has own animals, supplies, equipment and animal care staffing Direct costs Answer Salaries, fringe benefits, equipment, supplies, and operational exprenses (depend on # animals being housed) Indirect Costs Answer physical plant, energy costs, depreciation, heating rooms Cost Centers Answer Cage washing, laboratory services Why is cost accounting important? Answer Individulal costs used for projecting future cost; comparisons reveal areas in which costs can be reduced; per diem components (expense to maintain 1 animal for 1 day) Calculating per diem charges Answer Internal direct costs are incurred for the husbandry of animals and for other services provided by the animal facility for the benefits of special projects If total costs of maintaining a colony of cats is $27,375 over a 1 year period and the average daily census is 25 cats, what is the per diem charge needed to recover these costs? Answer Divide $27,375 by 365 and multiply the result by 25 = 3.00 What are somethings to keep in mind when considering injection techniques? Answer Injections with needles and syringes used to administer various substances; syringe construction and sizes; considerations for blood withdrawal; proper disposal, common routes of injection Intramuscular Injection (IM) Answer Generally given in hind legs (most desirable site is large muscle mass); alternative site are the muscles posterior to femur; do not inject too much material Intraperitoneal Injection (IP) Answer Given in lower right quadrant of abdomen (avoid bladder and cecum); Slight resistance may be encountered; Aspirate prior to injection (Yellow= bladder; greenish = intestine or cecum; Blood = blood vessel)