BIOD 171 Module 3 Exam
Question 1
True or False: A nanometer (10-9) is longer than a micrometer (10-6).
True
Co
orrrec
ct! False
A nanometer is 1,000 times smaller than a micrometer.
Question 2
Resolution and contrast are two critical factors that influence your ability to see an objec
Your Answer:
In Module 3, contrast is when the difference in the absorbance of light is conferred betwe
contrast is limited, it is hard to visualize multiple objects within the background. Alternati
referencing resolution, the distance between two objects and how they're seen as separ
studied. Low resolution occurs when multiple objects appear as one instead of separate
Resolution refers to the distance between two objects at which the objects stil
separate. Poor or low resolution means two (or more) objects may appear as o
Contrast is the difference in light absorbance between two objects. Poor contr
background and makes the visualization of multiple objects difficult. For instan
identify 2 dark colored objects at night (low light = low contrast) versus the sam
the middle of a sunny afternoon (bright light against 2 dark objects = high con
Question 3
aded by 1000008493198
, Assuming a fixed ocular, identify the part of the microscope you would adjust to enhance
a sample.
Co
orrrec
ct! Objective
Only the oculars (eyepiece) and the objectives contribute to the magnification of the sample
eyepiece is fixed, only the objectives could be altered.
Condenser
Iris diaphragm
Eye piece
Question 4
What is the total magnification (relative to your eye) of a sample imaged with a 20x obje
eyepiece? Show your math.
Your Answer:
TM is calculated by multiplying the power of the objective lens being used to the power
example -- if the objective lens being used is 60x, then the total magnification is 10 x 60
would appear 600 times larger.
20 x 15 = 300x magnification
Question 5
True or False: A cell that is adherent, flat (thin), and unstained is easily identified using b
microscopy.
, Co
orrrec
ct! Skin cell with diameter of 1500 μm
Bacteria with diameter of 24 μm
B and D. The unaided eye can, on average, clearly resolve objects > 100 μm
Question 7
Label the following unmarked microscope components (numbered arrows) by matching
components provided (letters).
A. Stage
B. Fine Adjustment Knob
C. Iris Diaphragm
D. Neck
E. Condenser Lens
F. Eyepiece
G. Objective
Question 1
True or False: A nanometer (10-9) is longer than a micrometer (10-6).
True
Co
orrrec
ct! False
A nanometer is 1,000 times smaller than a micrometer.
Question 2
Resolution and contrast are two critical factors that influence your ability to see an objec
Your Answer:
In Module 3, contrast is when the difference in the absorbance of light is conferred betwe
contrast is limited, it is hard to visualize multiple objects within the background. Alternati
referencing resolution, the distance between two objects and how they're seen as separ
studied. Low resolution occurs when multiple objects appear as one instead of separate
Resolution refers to the distance between two objects at which the objects stil
separate. Poor or low resolution means two (or more) objects may appear as o
Contrast is the difference in light absorbance between two objects. Poor contr
background and makes the visualization of multiple objects difficult. For instan
identify 2 dark colored objects at night (low light = low contrast) versus the sam
the middle of a sunny afternoon (bright light against 2 dark objects = high con
Question 3
aded by 1000008493198
, Assuming a fixed ocular, identify the part of the microscope you would adjust to enhance
a sample.
Co
orrrec
ct! Objective
Only the oculars (eyepiece) and the objectives contribute to the magnification of the sample
eyepiece is fixed, only the objectives could be altered.
Condenser
Iris diaphragm
Eye piece
Question 4
What is the total magnification (relative to your eye) of a sample imaged with a 20x obje
eyepiece? Show your math.
Your Answer:
TM is calculated by multiplying the power of the objective lens being used to the power
example -- if the objective lens being used is 60x, then the total magnification is 10 x 60
would appear 600 times larger.
20 x 15 = 300x magnification
Question 5
True or False: A cell that is adherent, flat (thin), and unstained is easily identified using b
microscopy.
, Co
orrrec
ct! Skin cell with diameter of 1500 μm
Bacteria with diameter of 24 μm
B and D. The unaided eye can, on average, clearly resolve objects > 100 μm
Question 7
Label the following unmarked microscope components (numbered arrows) by matching
components provided (letters).
A. Stage
B. Fine Adjustment Knob
C. Iris Diaphragm
D. Neck
E. Condenser Lens
F. Eyepiece
G. Objective
