ASCP CG Certification Practice
What is the best agent to use against mycoplasma? - Answer- BM cyclin - a combination of two antibiotics (tiamulin and minocycline) that effecticely targets mycoplasma growth For successful harvest, each new batch of hypotonic solution should be evaluated by: - Answer- comparing the new lot to a lot already in use Colagenase, pronase, and trypsin are: - Answer- Proteolytic enzymes - break down tissues into single-cell suspensions When harvesting peripheral blood cultures, asceptic technique no longer matters following incubation with: - Answer- Colcemid - mitotic spindle poison that halts cells at metaphase Samples that arrive at the cytogenetics lab should first be: - Answer- Logged in to the system Phytohemagglutinin (PHA) stimulates which type of cells into division? - Answer- T-cell lymphocytes To avoid culture failure, monitoring reagents is crucial. When logging a culture failure, what is the MOST important to document? - Answer- Expiration dates of reagents used Which type of microscope is used when cleaning a CVS (chorionic villus) sample? - Answer- Dissection Which cell type is most problematic if found in a CVS (chorionic villus) sample? - Answer- Decidua (maternal tissue) When looking at a slide under a phase contrast scope and a lot of cytoplasm is still seen around the mets, what is the most likely cause? - Answer- innappropriate slide drying time What will help prevent microbial contamination and cross contamination of cultures? - Answer- Asceptic technique When there are not enough mets to complete the study of a patient/case, what needs to be done next? - Answer- prepare additional slides from any remaining sample When looking at a dropped slide under a phase scope and mets are very spread out and sparse, what could help fix the issue? - Answer- Increading the drying time by using warm slides
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