BIOD171 Microbiology Lab Notebook LATEST

Title: B, Lerner #PL01 Introduction to Medical Microbiology Per graded lab, Your title should be BL01 Intro... You don't need to use PL anymore. Objective: To understand course objectives, basic equipment used, as well as general lab safety while working in a lab setting. Also, to understand how to create a lab notebook that is well organized. All documentation should be recorded in a lab notebook while completing a lab. Including the title, the objective (personal benefit to recall what was done during that lab), the procedure (step by step instructions of what was done in that lab, along with any deviations from the standard protocol), the notes (helpful information that does not fall into the procedure section but that one would want to remember from the lab), and the results (the last section which summarizes the findings during the lab and the main points of the lab). Procedure: 1. Step by step protocol recorded here a. Including: i. What chemicals were used ii. growth conditions iii. temperature iv. what type of plates were used? 2. Indicate any deviations that may occur during the experiment a. Write any deviations in red (anything that is not standard protocol) that occurred i. Deviations - something that absolutely stands out and is different than the standard protocol Notes: Additional (helpful) information, things that do not fall directly in the procedure • Course Objectives – o Cultivation of samples (growth conditions) ▪ equipment used ▪ the cultivation of growth conditions of the samples in the lab settings o Identification of samples (biochemical assays) ▪ tests available ▪ to look and determine what bacteria or what pathogens a patient may have o Evaluation of samples (microscopy) ▪ visualization and recognition of key variables (characteristics) of each pathogen • Basic Equipment o Cleaning – working in a sterile environment to ensure everyone stays healthy ▪ Autoclave – device that uses heat, pressure, and steam equipment in a lab to sterile equipment and also any samples that you might need to kill after using them • Temperature – above 125 degrees C • Come in different sizes • Does not just use dry air, uses hot steam o steam is much more efficient than dry air to sterile something o Dry hot air takes a couple hours to achieve a sterile condition o Hot steam only will take a couple mins to achieve a sterile environment o Need to be very careful when opening autoclave when finished using it because very hot steam will come out, so you need to make sure it has depressurized before you open it and get samples out and use thermal gloves o Growing ▪ Fixed incubator – • chamber • door is airtight, seal, and has a metal door to keep conditions dark • Temperature – 37 degrees C ▪ Shaker incubator – • when you want to take a growth media (liquid nutrient broth) to inoculate your bacteria • put it into the shaker and it actually rotates and shakes up the media in a swirling fashion to help aerate it (providing oxygen to the sample) which helps the bacteria grow under normal conditions • Temperature 37 degrees C o Visualizing ▪ Microscopy o Storing ▪ Refrigerator • Very helpful if you do not finish everything in one day • Temperature 4 degrees C o At 4 degrees you slow the bacterial growth which preserves your samples and helps keep them long term o When putting test tubes in the refrigerator you want to wrap parafilm around it to keep them airtight • General Lab Safety o Never eat or drink in the lab ▪ Contamination risks o Use personal protective equipment (PPE)