ASCP MB question and answers 2022

ASCP MB question and answers 2022E. coli RNA Polymerase II DNA template, product: mRNA RNA Polymerase I DNA template Location: Nucleous Products: 18s, 5.8s, 28s rRNA RNA Polymerase II DNA template Location: Nucleus Products: mRNA, snRNA RNA Polymerase III DNA template Location: Nucleus Product: tRNA, 5s rRNA, snRNA Mitochondrial RNA Polymerase DNA template, product: mRNA Mammalian DNA polymerase α DNA template, product: primers HCV RNA Polymerase RNA template, product: viral genome Dengue virus RNA Polymerase RNA template, product: viral genome Poly A Polymerase No template, product: Poly A tail 230 wavelength Contamination of organic compounds 270 wavelength Phenol contamination 280 wavelength Protein contamination 330 wavelength Particulate matter contamination Southern Blot DNA Target Nucleic acid probe Purpose: Paternity testing, gene discovery and mapping, mutation analysis and ID, gene structure Northern Blot RNA Target Nucleic acid probe Purpose: Determine transcript structure, processing, and gene expression Western Blot Protein Target Protein probe Purpose: Protein processing, gene expression Southwestern Blot Protein target DNA probe Purpose: DNA binding proteins, gene regulation Eastern blot Protein target Protein probe Purpose: Modification of Western blot using enzymatic detection (protein processing, gene expression), detection of specific agriculturally important proteins Far-eastern blot Lipid target No probe Purpose: Transfer separated lipids to membranes for analysis by mass spectometry PCR Primers Direct DNA synthesis to desired region PCR: dATP, dCTP, dGTP, dTTP Building blocks that extend primers PCR KCl Salt for optimal hybridization of primers to template PCR: Tris buffer Buffer to maintain optimal pH for enzyme reaction PCR: MgCl2 Divalent cation, required by enzyme PCR Polymerase Polymerase enzyme that extends the primers, adds the dNTPs PCR: copies of template Sample DNA that is being tested Denaturation temps/time 90-96, 20-60 sec Annealing temps/time 50-70, 20-90 sec Extension temps/time 68-75/10-60 sec + gain - loss del deletion der derivative chromasome dup duplication ins insertion inv inversion i, iso isochromosome mat maternal origin