BIOD 171 Microbiology Lab 8 Exam Portage Learning All Answers Correct Rated A+

NT, #8, Biochemical Assays for Bacterial Antigen Detection Objective1. To understand the assays available for detecting bacterial or viral antigens 2. Antigen- antibody generating antigen- foregin in system requiring antigen to detect Notes1. ELISA a. Enzyme- Linked Immunosorbent Assay, utilizes antibodies and a colorimetric readout to indicate an antigen of interest b. 96 Well PLATE- sandwich c. Each well already labeled with antibody – BLUE y shaped structure d. Each well add patient sample- serum or cultures e. B= bound by antibody f. B to C= wash step (remove any antigen unbound) g. C= capture of antigen or antibody h. D- incubation A-D no color i. E- tag is enzyme or substrate will act once activate j. E= final/ activated getting colormetric change- dark color, yellow or or wont change Get All the Correct Lab Answers