C•\,,,.
•
rrc,~le pnase (
i~ tM iolvrnt-
u1td j-Ol ttitin<J, cl1,1e1 in
~d. c10\flt1, 1ncdicinc1
<;~eh
i l'\vi<;\'o1c
Ullh~
\
l 'cc,n
0,,
tnt'f\ 11'\0 l'\Q
c.o lC1ured s~oll ore
l'f\Or1ttd In COit tnt~ f-OdC
lo <.
o.mr ,,.
(
____.,,<;iomechtrt1ico11 \'\t<O
coo 01,0 be id,nlifi<d ur,n~ i11 Rf
dnt{lne,c mortd bid ~am~
~-:: di110ncr n111¼'.d bl,! ~€]>,tn\
r\ ufOUS ( q_u1c~er thon po.pn ( 1 ro be de\-tlope d
4 parallel pol~pepbdHhalnr ht\_d tll~thtr \:)i uorr-\in\.l I' Chnirno.kl~roph'A, )
l\ f\t-.,(r , / 1
I
4~tm 1oni -rope-u~nnuc1ut-t1 W1th \r\iqh tcmtlrnrcri~t\1 TLC ¼-ton tie uwH<1v C.H RoMATOCd~lH,1\-::t\'\ii-- 'iPrOl!C0 1'1'\H\ t\.
10.l.\'1 poper 0r p1a\e cl-ionlcO.l thO.t
imoluble 1n wnHf .;
~:;t \
t ."
l{ ~ mo~ w'o1tt.1nc,u
4 \<J:.RATiN - hoc t\ IQ(~ pro-ponrCM..ct c.~~wnt W\'\,cn 'ifQbono1" ( lS0 lvtnt~~ II (.QU1tr (IJ11lUY <.\-\Onf
l'i'\ ti.n<.ed Mih penci I
r-{1u1t1 ,nHn:in~d.Hu4icle boclq.-t1 ~rmlni t1.lonca, t~o~~\"a~~~nt
· • • I ~Ovu ()(\Cl~ n~t - ,r ._ .
i~1e-x.,bte rnot-eno , p\o\tn,/ 10~ - ·• - : ·• ( . 1.·"' --, , ,,. · 30 <:,hQpt pro~tin i~
4 l-{Mr tlitUl~debondt, the ll\Qtf f"x,blC <a i
ffi()j(IHl.lnld \)~ \.\ 'oon<h,
L, Elf-l~TI N- prrnnt in 0\11eou wQ\11 blood vt!lfll to ~e~ratc, c.hernica\r amiat
L
idfl"\b~e 1 tMm
\\\\d~nab!c ,nttroc\1CIU-t
on,s '""" to 'XPUnd > <0n1<act to on~;no1 Site \ , """ on mo-tm<n, SI- 101 uQu;d -m,,;« ,no«
4 <\.UClttma,,. prottio mo.dt ':ihtttn~ tropotllltbn tnruurr,.o.~tc\iuY'r" ~\J~,{n - (\rtU' o.i~Ull-idtC\, bond•
\.>iO\"\e
tht ~trc~n
4 CGLLAGEN -c:.onnecb--c trnue ih tendon,, nuvo1.u S'4\ttrt1 \to.\1~::'t , pepbctr, Cltt pol(!mfl'\ YViadt up
'\
I O(<.Ul\ \:)t\-V.U.I\ '2
fl"•""
C, :i pol',"' pt;d e, "<Ound h, o tn pl< heV, - ,op,. V«
~l"'-"'!,~rdo.minoOcid~Crl'(t\N£ .
!tn,ci,,«e
PROTE\NS
- )
. .
"\ """'"' Qtid In<> \t<UI"
, t - C.-H
Clf,H int\
Lo, \\llfUt-C.Ol\\-ain.in~
Ui \,\ bcn·,o~ ~nwetn Chall\\ ~lYY\ 'S,\-0.<!~ercd erot- C.Qn ~Qi(\ end l"o
cr : \ PtC\(:1(\I (.l)('\i1\\ 1or l'Y)()'.-< I Otnino oc,ds ,
tnd , l"<m;"'¼ ""I ~••" to11t6 wi,oco11o<f n • "•"· un• to '"'""" ~"'"
4 pt0\.iM. is h\!(\rtil.~pvrunt ttpel adtlini 'ito.bllih~
4 ~~1nom i-o reanni
I:'., , .-.- -,,-C Cr ------- \ ~m,ed
.
( rnmromolecu1c1
condtruotiOV\
A. ,rt;~
·po I~ P<Pbd< r Qrrun'f d •• tom pit,
om.ino aud
~ne1 on
r-e c.ar'--•\I
·i 1we1t•
,- \ 0•• t:... ,
in
11_~ • t{Octon1 '6?\'ptide'o?nd1 .
0.dd an equal volUl'\'\l
KCtcum to Cl \Qmpit 'iOIUti«M
Siut{t °t:)~t~n 1nd ll/\cilJCll \
C.ll'n lno OC((\ roolecv1e1
~ \ bCtWW'\,~
_-u \ Cl-Ttll1A ~"11• ~ ~
rrn~m~~l'(ru~ on 1OmiM Oc.id
.non'-''',
'l, " ' ' Ql"(\jn(.I
r 4 t l'-h• '2. -ii - - -.!J --:in tt"lt. OtnC< ~~rN\I (.\ OJ ,..,.,u"'-"
Should turn lo1uc to purple ; 4 p roduCQ.1 1,-1a~r
4 Sfl\')<:ritOI ShO.p( COUI td b~
~~pli\ bOCI<. intc, '2. t\f(\j"C oc.id~ If\ 0. nio~\~s.\\ rtO.C.1JOf\
tl~htl~ f<ild,d pol~pep11de cho',nt
n~drtJ?I.ObiC(3t0\IPI ()\ in'i(d("'
n~drtrprulic onouts,de mo.ti"C! thfl'V\ 111111 •
~lllUble In i--,Qt<r 4 molt arn;no ac1d1 ~n to a d.ll)(p\Jde
I,~-~. tror,!iport -pro~tn\, tnt~nw.1 4 o. Prt>ttin \ll ffi()tt pol~ptptide thOim ~\ded IY\tt> tl N~hll!,
.. Clnd h0ml\ll'\tl
4111m.iu~ - t\alman~ lrwotvtd in
<:.pc ~c aD c. \'\Ctpe
PnrnQ.\'\l: 't,imp1e tonta Cl"IC\.iri QT'(\jY\~ Q.ddl with Y\C ir.t\'amcltC.Ular
4~\obl.110.1 pn:.ttin1 witn Cl non-!)rotein proithetic iroup
t{(!,ulo.\in\ \-ilo\ld Ck\uto1e col"lccl'\t1a\l'(f(I bonO\ er int'lract,lonJ
1-1 AEtvt0GL08t N - C\lJQt(mQ"'4- lnodt pol~pep\::icte1, 2 (J..
~rrQl"I\Pomd ,n 1:i1ood1trtafYI ro S(c.at'(io·c,f \\ bonds ~'Im c.o.ulin~ \ht MlllttlJ\t H1 c~\\ \. e1. hel.ix) ot
QI)(\ 2 P.. j_Ubun,ts - {Och conta,n,
a pr oithftiT hQon group
need to be solubtc
Iron .ll. iMl bl nd rtvt.nl bl\!, tCl e't~~n ~d ,~picoted \nl?ttt')
4 CATALASE - C¼Qttman. CQO\Q.11'\in~ LI prolthetic harm ~rOl.JP{ . L,no.1 o 1ncci1t sna~t tH Ten:inrA: h~drophllidht!dJOPhllbiC bonc\1, btrnc.lr, ionic ?.di!.u\~dc
m\\l \t)tc.J!-{t rtetptori Ct\ PIOIMO MtmblOJ'lt '«inch no1d1 rno1cc.o1e1 in \hap('. ·
4iron1L ICM Qllow1 coto101e tointero.ct Y'rith h~ro~n Pet'O);fciA
to '$peed uP In 'ortotdorn, Ouo.ttmaf\l: ,o.rM 0.1 HC'd<l"i, b.t\ '2 pa\~pc~de (ho.ini lnttCUnud
b~µruduc.t~ rne.tubolifl\-'l t:JU\ dornuwnq to
WI~ iq- it CICCUff\\.llClTCl
•
rrc,~le pnase (
i~ tM iolvrnt-
u1td j-Ol ttitin<J, cl1,1e1 in
~d. c10\flt1, 1ncdicinc1
<;~eh
i l'\vi<;\'o1c
Ullh~
\
l 'cc,n
0,,
tnt'f\ 11'\0 l'\Q
c.o lC1ured s~oll ore
l'f\Or1ttd In COit tnt~ f-OdC
lo <.
o.mr ,,.
(
____.,,<;iomechtrt1ico11 \'\t<O
coo 01,0 be id,nlifi<d ur,n~ i11 Rf
dnt{lne,c mortd bid ~am~
~-:: di110ncr n111¼'.d bl,! ~€]>,tn\
r\ ufOUS ( q_u1c~er thon po.pn ( 1 ro be de\-tlope d
4 parallel pol~pepbdHhalnr ht\_d tll~thtr \:)i uorr-\in\.l I' Chnirno.kl~roph'A, )
l\ f\t-.,(r , / 1
I
4~tm 1oni -rope-u~nnuc1ut-t1 W1th \r\iqh tcmtlrnrcri~t\1 TLC ¼-ton tie uwH<1v C.H RoMATOCd~lH,1\-::t\'\ii-- 'iPrOl!C0 1'1'\H\ t\.
10.l.\'1 poper 0r p1a\e cl-ionlcO.l thO.t
imoluble 1n wnHf .;
~:;t \
t ."
l{ ~ mo~ w'o1tt.1nc,u
4 \<J:.RATiN - hoc t\ IQ(~ pro-ponrCM..ct c.~~wnt W\'\,cn 'ifQbono1" ( lS0 lvtnt~~ II (.QU1tr (IJ11lUY <.\-\Onf
l'i'\ ti.n<.ed Mih penci I
r-{1u1t1 ,nHn:in~d.Hu4icle boclq.-t1 ~rmlni t1.lonca, t~o~~\"a~~~nt
· • • I ~Ovu ()(\Cl~ n~t - ,r ._ .
i~1e-x.,bte rnot-eno , p\o\tn,/ 10~ - ·• - : ·• ( . 1.·"' --, , ,,. · 30 <:,hQpt pro~tin i~
4 l-{Mr tlitUl~debondt, the ll\Qtf f"x,blC <a i
ffi()j(IHl.lnld \)~ \.\ 'oon<h,
L, Elf-l~TI N- prrnnt in 0\11eou wQ\11 blood vt!lfll to ~e~ratc, c.hernica\r amiat
L
idfl"\b~e 1 tMm
\\\\d~nab!c ,nttroc\1CIU-t
on,s '""" to 'XPUnd > <0n1<act to on~;no1 Site \ , """ on mo-tm<n, SI- 101 uQu;d -m,,;« ,no«
4 <\.UClttma,,. prottio mo.dt ':ihtttn~ tropotllltbn tnruurr,.o.~tc\iuY'r" ~\J~,{n - (\rtU' o.i~Ull-idtC\, bond•
\.>iO\"\e
tht ~trc~n
4 CGLLAGEN -c:.onnecb--c trnue ih tendon,, nuvo1.u S'4\ttrt1 \to.\1~::'t , pepbctr, Cltt pol(!mfl'\ YViadt up
'\
I O(<.Ul\ \:)t\-V.U.I\ '2
fl"•""
C, :i pol',"' pt;d e, "<Ound h, o tn pl< heV, - ,op,. V«
~l"'-"'!,~rdo.minoOcid~Crl'(t\N£ .
!tn,ci,,«e
PROTE\NS
- )
. .
"\ """'"' Qtid In<> \t<UI"
, t - C.-H
Clf,H int\
Lo, \\llfUt-C.Ol\\-ain.in~
Ui \,\ bcn·,o~ ~nwetn Chall\\ ~lYY\ 'S,\-0.<!~ercd erot- C.Qn ~Qi(\ end l"o
cr : \ PtC\(:1(\I (.l)('\i1\\ 1or l'Y)()'.-< I Otnino oc,ds ,
tnd , l"<m;"'¼ ""I ~••" to11t6 wi,oco11o<f n • "•"· un• to '"'""" ~"'"
4 pt0\.iM. is h\!(\rtil.~pvrunt ttpel adtlini 'ito.bllih~
4 ~~1nom i-o reanni
I:'., , .-.- -,,-C Cr ------- \ ~m,ed
.
( rnmromolecu1c1
condtruotiOV\
A. ,rt;~
·po I~ P<Pbd< r Qrrun'f d •• tom pit,
om.ino aud
~ne1 on
r-e c.ar'--•\I
·i 1we1t•
,- \ 0•• t:... ,
in
11_~ • t{Octon1 '6?\'ptide'o?nd1 .
0.dd an equal volUl'\'\l
KCtcum to Cl \Qmpit 'iOIUti«M
Siut{t °t:)~t~n 1nd ll/\cilJCll \
C.ll'n lno OC((\ roolecv1e1
~ \ bCtWW'\,~
_-u \ Cl-Ttll1A ~"11• ~ ~
rrn~m~~l'(ru~ on 1OmiM Oc.id
.non'-''',
'l, " ' ' Ql"(\jn(.I
r 4 t l'-h• '2. -ii - - -.!J --:in tt"lt. OtnC< ~~rN\I (.\ OJ ,..,.,u"'-"
Should turn lo1uc to purple ; 4 p roduCQ.1 1,-1a~r
4 Sfl\')<:ritOI ShO.p( COUI td b~
~~pli\ bOCI<. intc, '2. t\f(\j"C oc.id~ If\ 0. nio~\~s.\\ rtO.C.1JOf\
tl~htl~ f<ild,d pol~pep11de cho',nt
n~drtJ?I.ObiC(3t0\IPI ()\ in'i(d("'
n~drtrprulic onouts,de mo.ti"C! thfl'V\ 111111 •
~lllUble In i--,Qt<r 4 molt arn;no ac1d1 ~n to a d.ll)(p\Jde
I,~-~. tror,!iport -pro~tn\, tnt~nw.1 4 o. Prt>ttin \ll ffi()tt pol~ptptide thOim ~\ded IY\tt> tl N~hll!,
.. Clnd h0ml\ll'\tl
4111m.iu~ - t\alman~ lrwotvtd in
<:.pc ~c aD c. \'\Ctpe
PnrnQ.\'\l: 't,imp1e tonta Cl"IC\.iri QT'(\jY\~ Q.ddl with Y\C ir.t\'amcltC.Ular
4~\obl.110.1 pn:.ttin1 witn Cl non-!)rotein proithetic iroup
t{(!,ulo.\in\ \-ilo\ld Ck\uto1e col"lccl'\t1a\l'(f(I bonO\ er int'lract,lonJ
1-1 AEtvt0GL08t N - C\lJQt(mQ"'4- lnodt pol~pep\::icte1, 2 (J..
~rrQl"I\Pomd ,n 1:i1ood1trtafYI ro S(c.at'(io·c,f \\ bonds ~'Im c.o.ulin~ \ht MlllttlJ\t H1 c~\\ \. e1. hel.ix) ot
QI)(\ 2 P.. j_Ubun,ts - {Och conta,n,
a pr oithftiT hQon group
need to be solubtc
Iron .ll. iMl bl nd rtvt.nl bl\!, tCl e't~~n ~d ,~picoted \nl?ttt')
4 CATALASE - C¼Qttman. CQO\Q.11'\in~ LI prolthetic harm ~rOl.JP{ . L,no.1 o 1ncci1t sna~t tH Ten:inrA: h~drophllidht!dJOPhllbiC bonc\1, btrnc.lr, ionic ?.di!.u\~dc
m\\l \t)tc.J!-{t rtetptori Ct\ PIOIMO MtmblOJ'lt '«inch no1d1 rno1cc.o1e1 in \hap('. ·
4iron1L ICM Qllow1 coto101e tointero.ct Y'rith h~ro~n Pet'O);fciA
to '$peed uP In 'ortotdorn, Ouo.ttmaf\l: ,o.rM 0.1 HC'd<l"i, b.t\ '2 pa\~pc~de (ho.ini lnttCUnud
b~µruduc.t~ rne.tubolifl\-'l t:JU\ dornuwnq to
WI~ iq- it CICCUff\\.llClTCl
