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BIOL 3311 - Test 2 Practice Test Study Guide - Fall 2020/21

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Several sequences from different species that align to one another may be Homologous sequences, orthologous sequences 2. BLAST stands for Basic Local Alignment Search Tool 3. Two equal peaks, larger than the others, in a FinchTV chromatogram indicates Two of the bases can be ruled out as being located at that position 4. An acceptable E-value for a nucleotide sequence alignment is 1e-6 or less and identity of 70% or more 5. An acceptable E-value for an amino acid sequence alignment is 1e-3 or less and identity of 25% or more 6. How many common amino acids are found in Eukaryotes? In eukaryotes, there are only 21 proteinogenic amino acids, the 20 of the standard genetic code, plus selenocysteine. 7. Which of the following is a start codon found in a fasta file? Met/AUG/ATG 8. What are the three stop codons? UAG, UAA, and UGA 9. Which of the following are advantages to using Flybase over other search tools? Flybase is simplified/orientated for fly work. All fly data is compiled here including literature, orthologs etc. (all the tabs on the right side) , Gbrowse, Jbrowse 10. When two organisms are very far apart on the tree of life you should Expect less homology, (phylogenetic trees), (Have an earlier common ancestor)

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