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Measuring Gene Expression: Theory, Techniques and Application

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2021/2022 Written for academic year
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Techniques and Applications in Biomedical Science (6H5Z1036)
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Lecture notes on Measuring Gene Expression: Theory, Techniques and Application

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https://www.youtube.com/watch?v=1kvy17ugI4w
https://www.youtube.com/watch?v=0ATUjAxNf6U https://www.youtube.com/watch?v=G2Z1wuEOZUU
https://www.promega.co.uk/resources/multimedia/reporter-assays-and-transfection/introduction-to-reporter-gene-
assays/

Measuring Gene Expression: Theory, Techniques and Application




Regulation of Gene Expression: Transcription




The Fireflies:

, - Order Coleoptera: Family Lampyridae
- 2,000 described species
- Produce bioluminescent light from the lower abdomen during twilight to attract mates or prey
- Light may be yellow, green, or pale red, with wavelengths from 510 to 670 nm

The Chemistry of Bioluminescence:




Reporter Gene Assays: Principle II




Reporter Gene Assays: Applications
- Understanding promoter structure
- Analysis of transcription factor structure
- Activation of cell receptors
- Studying effects of drugs/treatments on gene expression

Types of eukaryotic RNA:

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The Manchester Metropolitan University
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January 18, 2022
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2021/2022
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Class notes
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Dr james pritchett
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Subjects

  • human
  • bioscience
  • biomedical
  • gene expression

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