Written by students who passed Immediately available after payment Read online or as PDF Wrong document? Swap it for free 4.6 TrustPilot
logo-home
logo
  • The Manchester Metropolitan University (MMU)
  • The Manchester Metropolitan University
  • Techniques and Applications in Biomedical Science (6H5Z1036)
To add items to your wishlist, you must be logged in
Document preview thumbnail
Preview 1 out of 3 pages
Class notes

Measuring Gene Expression and Western Blotting

0.0
(0 reviews)
- sold
3 pages
2021/2022 Written for academic year
Institution
The Manchester Metropolitan University (MMU)
Course
Techniques and Applications in Biomedical Science (6H5Z1036)
Document preview thumbnail
Preview 1 out of 3 pages

Lecture notes on Measuring Gene Expression and Western Blotting

Content preview

https://www.youtube.com/watch?v=GJJGNOdhP8w
https://www.youtube.com/watch?v=IoVzpL_heFo

Protein level - Western blotting

Western Blotting (Immunoblotting): Principles
A laboratory method using antibodies to detect
specific protein molecules in a mixture of proteins.
Combines:
- Separation of proteins in a semi-solid
Polyacrylamid gel (PAG) using electrophoresis
- Electroblotting of the separated proteins onto a
solid membrane (nitrocellulose or
polyvinylidene fluoride (PVDF)
- Detection of target proteins by specific
antibodies

Gel Electrophoresis Formats




Sample preparation for SDS-PAGE
- Reducing agents (e.g. 2- mercaptoethanol): reduce
disulphide bridges and dissolve tertiary and quaternary
structures
- SDS: attaches to protein molecules, charges them
negatively and linearises the protein chains

SDS-PAGE

Document information

Study
The Manchester Metropolitan University
Uploaded on
January 18, 2022
Number of pages
3
Written in
2021/2022
Type
Class notes
Professor(s)
Dr james pritchett
Contains
All classes
Report Copyright Violation
$14.62
Wrong document? Swap it for free Within 14 days of purchase and before downloading, you can choose a different document. You can simply spend the amount again.
Written by students who passed
Immediately available after payment
Read online or as PDF
Seller avatar
Reputation scores are based on the amount of documents a seller has sold for a fee and the reviews they have received for those documents. There are three levels: Bronze, Silver and Gold. The better the reputation, the more your can rely on the quality of the sellers work.
polinalobacheva
3.8
(5)
Sold
12
Followers
9
Items
138
Last sold
4 year ago
Available in package deal
Product Image
Package deal
Techniques and Applications in Biomedical Science
-
-
Sold
17
item
Jan 2022
$248.46
$140.01
You save 44% More Info

Browse related subjects

Subjects

  • human
  • bioscience
  • biomedical
  • gene expression
  • gene
  • western blotting
  • blotting
  • western

Why students choose Stuvia

Created by fellow students, verified by reviews

Quality you can trust: written by students who passed their tests and reviewed by others who've used these notes.

Didn't get what you expected? Choose another document

No worries! You can instantly pick a different document that better fits what you're looking for.

Pay as you like, start learning right away

No subscription, no commitments. Pay the way you're used to via credit card and download your PDF document instantly.

Student with book image

“Bought, downloaded, and aced it. It really can be that simple.”

Alisha Student

Working on your references?

Create accurate citations in APA, MLA and Harvard with our free citation generator.

Learn more Create citation
Working on your references?

Frequently asked questions