Microbiology Lab Master Guide

When you finish using the compound brightfield microscope, what should you have your lab instructor do? Inspect your microscope to see if it is properly cleaned You are allowed to ask your lab instructors and TAs any questions about lab related topics. True What do you have to do when you finish using the compound brightfield microscope? Clean the microscope and ask your lab instructor to inspect it Where should you dispose of paper towels used to clean your bench at the beginning and end of the lab? In the regular trash can in the lab You are allowed to chew gum in lab. False You are allowed to eat and drink water in lab. False Which of the following slides will you view in lab period 1? All are correct (protozoa, wet mount of unstained microbes, prepared yeast, prepared bacteria) Which objective lenses will you be using in this lab period? 10X, 40X, 100X Which of the objective lenses on the compound light microscope must always be used with immersion oil? 100X Objective Lens You are sick and cannot go to lab, what do you need to do? Contact the doctor and get a doctor's note to minimize the risk of losing course points for missing a lab Give a doctor's note to your lab instructor when you are well and attending lab again You are required to wear closed toe shoes when attending the microbiology labs True You are required to tie your shoulder-length (or longer) hair back. True Module 1 Quiz: Emily, a student in a microbiology course, got ready to leave home for the third lecture and lab of the semester. She grabbed her backpack with all the needed material, her elastic band for to tie her hair back and her flip flops, and headed to school. What did the student forget? Her close toed shoes The lab instructor entered the lab, took a sip of her water bottle, and pulled up the PowerPoint presentation on the screen. She then quickly moved the water bottle so it faced the back of the computer instead of the lab, and then started the lab presentation. What should she have done differently? Drink from the water bottle before entering the lab and leave the water bottle outside the lab, before entering. Where should you dispose of used swabs in lab? Red biohazard bucket Which part of the microscope can be used to raise and lower the stage? The mechanical stage Which part of the microscope should not be necessary to use once the specimen is in focus using the 10X objective? The coarse focus adjustment knob When looking at a specimen using the the oil immersion objective lens the total magnification is what? 1000X Which of the following microscopes are light microscopes? Fluorescence microscope, phase-contrast, compound brightfield, darkfield Both E. coli and S. cerevisiae use flagella for motility. False