BIOS242_Week1LabWorksheet

There are four safety equipment items that a lab should have. Identify two of these four items. (2 pts) Biological Safety Cabinet, Eyewash and shower 2. Identify one of the three ways to keep your work area safe. (1 pt) Keep your workspace free of all unnecessary materials 3. There are five recommendations for dressing properly in a lab environment. Name two of these recommendations. (2 pt) Avoid wearing loose fitting items of clothing, and wear appropriate shoes ,sandals are not allowed. 4. There are several safety tips to protect one’s mucous membranes and broken skin. Identify one of the recommended tips. (1 pt) Don’t apply makeup or attempt to put on contacts. 5. When working in a microbiology lab, it is important to contain spills and discard materials properly. Describe how a spill should be cleaned up safely in a microbiology lab. (3 pts) Make sure you are wearing a lab coat, disposable gloves and safety goggles if needed. Soak a paper towel in the appropriate disinfectant and place on the spilled area. Working from the outside in clean the spill with fresh paper towel soaked in the disinfectant making sure to decontaminate any surface area that may have been affected by the spill. Throw away gloves and paper towels in biohazard bag and place materials in auto clave to sterilize them. 6. Describe the procedures that one must do before leaving the lab. (3 pts) Ensure that work area is clean and disinfected. Put away all materials used and wash your hands before leaving the lab. Section 2 Biochemical Tests 7. How do you know that no contamination occurred when you inoculated your culture? (1 pt) When there are no other bacteria visible on the culture after inoculation. 8. If the biochemical test requires an incubation of 24 hours, what do you need to do? (1 pt) iLab: Exploring the Microbiology Lab Page 1 This study source was downloaded by from CourseH on :43:14 GMT -06:00 Incubate the sample for 24hrs to allow bacterial growth. 9. If your biochemical test requires that a reagent is added, which tool do you need to use? (1 pt) You need to select the dropper from the tool list and select the reagent. 10. There are two ways to record the results of a biochemical test. Watch the video found in the Recording Results tutorial. What was the result of the acid from glucose test? (1 pt) The result was positive.