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GENETICS FINAL TEST SCRIPT
AUTHENTIC ITEMS PRECISE
RESPONSES VALIDATED


◉ A plasmid containing a foreign insert is known as a. Answer: two of
these choices


idk which 2


◉ A commonly used locus for selecting hybrid vectors is. Answer: two
of these choices


idk which 2


◉ DNA cloning can be carried out on blunt-ended DNA with. Answer:
all of these choices


-linkers
-blunt-end ligation
-poly-dA/poly-dT technique

, ◉ The key enzyme for blunt-end ligation is. Answer: T4 DNA ligase


◉ The form of DNA called cDNA refers to. Answer: complementary
DNA


◉ A laboratory method for transferring electrophoresed DNA is known
as. Answer: southern blotting


◉ Plasmids that allow the transcription and translation of their cloned
genes are called. Answer: expression vectors


◉ The technique of studying an extended length of DNA by way of
overlapping clones is called. Answer: chromosome walking


◉ For a prokaryotic plasmid to grow successfully in yeast, it must
contain. Answer: CEN


◉ A 650 bp piece of DNA is cut with a single restriction enzyme.
Fragments of 150*, 200*, and 300 bp are found. (Asterisks denote
radioactively labeled end pieces.) How many restriction sites are there?.
Answer: 2


figure out how to work this

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