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Microbiology Lab Practicum Review: Key Terms & Definitions Study Guide

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2026/2027

Institution Medical Microbiology

Course Medical Microbiology

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Microbiology Lab Practicum Review: Key Terms & Definitions Study Guide

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Microbiology Lab Practicum Review_
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Key 1Terms
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How do you write a scientific name in proper form? - Capitalize genus
- all the rest- lowercase
- underline if writing (italicize or underline if typing)

Parts of Microscope: Stage platform that holds specimen slide

Parts of Microscope: Substage Condenser focuses light from the lamp onto the specimen

Parts of Microscope: Course Adjustment Knob knob used to make larger changes in focus (to by used on 4x and 10x
only)

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,Microbiology Lab Practicum Review_
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Parts of Microscope: Iris Diaphragm mechanism that controls the amount of light that reaches the specimen

Parts of Microscope: 4x Objective Lens Scanning Lens - 40x total magnification (with a 10x ocular lens
microscope)

Resolution the ability to discern the different aspects of a specimen from eachother

How do you calculate magnification? Objective Mag x Ocular Mag = Total Mag

How do you prepare a heat-fixed smear of proper - Aseptically obtain a sample of bacteria and place it on a clean slide
density for staining a broth culture? - Spread liquid over a small area of the slide (coin sized)
- Place slide on slide warmer (if air drying - set out at room temp)
- Once smear is completely dry, pass over flame 3 times to heat fix

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& DefinitionsPage 3 of Page
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How do you prepare a heat-fixed smear of proper - Place a drop of DI (deionized) water on a clean slide
density for staining a solid, slant culture? - Aseptically obtain a sample of bacteria on loop and mix into drop of
water
- Spread liquid over a small area of the slide (coin sized)
- Place slide on slide warmer (if air drying - set out at room temp)
- Once smear is completely dry, pass over flame 3 times to heat fix

What are the steps for preparing a Gram Staining - Heat-fixed emulsion slide
Procedure - 1 drop of crystal violet (primary stain) for 45 seconds
- Gently rinse with distilled water into Tupperware container
- Cover smear with Iodine stain (mordant) for 15 seconds
- Gently rinse slide with distilled water
- Trickle down slide with 95% ethanol (decolorizer) until run off is clear
- Gently rinse with distilled water
- 1 drop Safranin (counterstain) for 30 seconds
- Rinse with distilled water
- Gently blot dry in a tablet of bibulous paper

What color is a gram-positive reaction? purple/blue

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Number of pages: 19
Written in: 2026/2027
Type: Exam (elaborations)
Contains: Questions & answers

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