UAMS- MICROBIOLOGY: REAGENTS
QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS 100%
CORECT!!!
What diagnostic enzyme test utilizes the reagent Tetramethyl-p-
phenylenediamine?
✔✔ Oxidase Test (detects bacterial cytochrome c oxidase activity)
What diagnostic reagent is used in the traditional tube method for testing
Indole production?
✔✔ Ehrlich’s / Kovac’s Reagent (Paradimethylaminobenzaldehyde / $p$-
DMAB) (detects indole produced by tryptophanase cleavage of tryptophan)
What antibiotic or chemical agent is contained in the "P Disk" used for
bacterial identification?
✔✔ Optochin (Ethylhydrocupreine hydrochloride) (used to differentiate
Streptococcus pneumoniae [susceptible] from other alpha-hemolytic viridans
streptococci [resistant])
40% KOH & Alpha-napthol -ANSWER✔✔Voges-Proskauer
Sulfanilic Acid & Dimethyl-alpha-naphthylamine -ANSWER✔✔Nitrate
Bacitracin -ANSWER✔✔A disk
Bile salts
(Sodium Desoxycholate) -ANSWER✔✔Bile solubility
Plasma -ANSWER✔✔Coagulase
QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS 100%
CORECT!!!
What diagnostic enzyme test utilizes the reagent Tetramethyl-p-
phenylenediamine?
✔✔ Oxidase Test (detects bacterial cytochrome c oxidase activity)
What diagnostic reagent is used in the traditional tube method for testing
Indole production?
✔✔ Ehrlich’s / Kovac’s Reagent (Paradimethylaminobenzaldehyde / $p$-
DMAB) (detects indole produced by tryptophanase cleavage of tryptophan)
What antibiotic or chemical agent is contained in the "P Disk" used for
bacterial identification?
✔✔ Optochin (Ethylhydrocupreine hydrochloride) (used to differentiate
Streptococcus pneumoniae [susceptible] from other alpha-hemolytic viridans
streptococci [resistant])
40% KOH & Alpha-napthol -ANSWER✔✔Voges-Proskauer
Sulfanilic Acid & Dimethyl-alpha-naphthylamine -ANSWER✔✔Nitrate
Bacitracin -ANSWER✔✔A disk
Bile salts
(Sodium Desoxycholate) -ANSWER✔✔Bile solubility
Plasma -ANSWER✔✔Coagulase