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Test Bank
by Robert McGehee and Brian Storrie
to accompany
The Cell: A Molecular Approach, Seventh Edition
Geoffrey M. Cooper and Robert E. Hausman
Chapter 8: RNA Synthesis and Processing
TEST FILE QUESTIONS
Multiple Choice
1. The prokaryotic cells most commonly used to study transcription and translation are
from
a. E. coli.
b. B. subtilis.
c. S. cerevisiae.
d. D. discoidium.
Answer: a
Textbook Reference: Transcription in Bacteria
Bloom’s Category: 1. Remembering
2. RNA polymerase differs from DNA polymerase in that it
a. synthesizes new strands of RNA in a 3ʹ to 5ʹ direction.
b. is a monomeric protein.
c. can synthesize a complementary strand without the two strands of DNA being
separated.
d. does not require a primer to initiate synthesis of RNA.
Answer: d
Textbook Reference: Transcription in Bacteria
Bloom’s Category: 2. Understanding
3. The DNA sequence to which an RNA polymerase binds to initiate transcription of a
gene is called a(n)
a. enhancer.
b. promoter.
c. polymerase-binding element.
d. origin of transcription.
Answer: b
Textbook Reference: Transcription in Bacteria
Bloom’s Category: 1. Remembering
4. The regions of the DNA where RNA polymerase binds can be identified by
a. restriction mapping.
b. PCR.
© 2016 Sinauer Associates, Inc.
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c. mutagenesis in the –35 and –10 promoter regions that inhibits transcription.
d. polymerase mapping.
Answer: c
Textbook Reference: Transcription in Bacteria
Bloom’s Category: 2. Understanding
5. Transcription is _______-dependent _______ synthesis.
a. DNA; DNA
b. DNA; RNA
c. RNA; DNA
d. RNA; protein
Answer: b
Textbook Reference: Transcription in Bacteria
Bloom’s Category: 2. Understanding
6. The role of the sigma () factor in prokaryote transcription is to
a. unwind the DNA during transcription.
b. direct RNA polymerases to bind to different promoter regions.
c. recognize the transcription initiation site.
d. terminate transcription.
Answer: b
Textbook Reference: Transcription in Bacteria
Bloom’s Category: 2. Understanding
7. Transcription of a bacterial gene is initiated
a. with RNA polymerase in the closed-promoter complex.
b. after release of the sigma () factor.
c. with RNA polymerase in the open-promoter complex.
d. Both b and c
Answer: c
Textbook Reference: Transcription in Bacteria
Bloom’s Category: 2. Understanding
8. Termination of transcription in E. coli is signaled by
a. formation of a stem-loop structure in the RNA.
b. binding of Rho protein to the end of the mRNA.
c. binding of a sigma () factor to the end of the mRNA.
d. an inverted GC-rich sequence followed by seven A residues.
Answer: d
Textbook Reference: Transcription in Bacteria
Bloom’s Category: 2. Understanding
9. The pioneering studies of gene regulation in E. coli were conducted in the 1950s by
a. Miller and Urey.
b. Watson and Crick.
c. Jacob and Monod.
© 2016 Sinauer Associates, Inc.
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The Cell 7e Ch08 Test Bank
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Test Bank
by Robert McGehee and Brian Storrie
to accompany
The Cell: A Molecular Approach, Seventh Edition
Geoffrey M. Cooper and Robert E. Hausman
Chapter 8: RNA Synthesis and Processing
TEST FILE QUESTIONS
Multiple Choice
1. The prokaryotic cells most commonly used to study transcription and translation are
from
a. E. coli.
b. B. subtilis.
c. S. cerevisiae.
d. D. discoidium.
Answer: a
Textbook Reference: Transcription in Bacteria
Bloom’s Category: 1. Remembering
2. RNA polymerase differs from DNA polymerase in that it
a. synthesizes new strands of RNA in a 3ʹ to 5ʹ direction.
b. is a monomeric protein.
c. can synthesize a complementary strand without the two strands of DNA being
separated.
d. does not require a primer to initiate synthesis of RNA.
Answer: d
Textbook Reference: Transcription in Bacteria
Bloom’s Category: 2. Understanding
3. The DNA sequence to which an RNA polymerase binds to initiate transcription of a
gene is called a(n)
a. enhancer.
b. promoter.
c. polymerase-binding element.
d. origin of transcription.
Answer: b
Textbook Reference: Transcription in Bacteria
Bloom’s Category: 1. Remembering
4. The regions of the DNA where RNA polymerase binds can be identified by
a. restriction mapping.
b. PCR.
© 2016 Sinauer Associates, Inc.
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c. mutagenesis in the –35 and –10 promoter regions that inhibits transcription.
d. polymerase mapping.
Answer: c
Textbook Reference: Transcription in Bacteria
Bloom’s Category: 2. Understanding
5. Transcription is _______-dependent _______ synthesis.
a. DNA; DNA
b. DNA; RNA
c. RNA; DNA
d. RNA; protein
Answer: b
Textbook Reference: Transcription in Bacteria
Bloom’s Category: 2. Understanding
6. The role of the sigma () factor in prokaryote transcription is to
a. unwind the DNA during transcription.
b. direct RNA polymerases to bind to different promoter regions.
c. recognize the transcription initiation site.
d. terminate transcription.
Answer: b
Textbook Reference: Transcription in Bacteria
Bloom’s Category: 2. Understanding
7. Transcription of a bacterial gene is initiated
a. with RNA polymerase in the closed-promoter complex.
b. after release of the sigma () factor.
c. with RNA polymerase in the open-promoter complex.
d. Both b and c
Answer: c
Textbook Reference: Transcription in Bacteria
Bloom’s Category: 2. Understanding
8. Termination of transcription in E. coli is signaled by
a. formation of a stem-loop structure in the RNA.
b. binding of Rho protein to the end of the mRNA.
c. binding of a sigma () factor to the end of the mRNA.
d. an inverted GC-rich sequence followed by seven A residues.
Answer: d
Textbook Reference: Transcription in Bacteria
Bloom’s Category: 2. Understanding
9. The pioneering studies of gene regulation in E. coli were conducted in the 1950s by
a. Miller and Urey.
b. Watson and Crick.
c. Jacob and Monod.
© 2016 Sinauer Associates, Inc.
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