and
IN
TRANSCRIPTION
INITIATION
ELKAR
genestructuretranscription
D
8R030NNNTOTANA3N
20JNRIN9
genestructure
2092OMIOA
proximal control elements corepromoter 8703Cl00bYd
6 910 NO1
ASZ
U1C1A site
PbIyCA)
-•
donnstream
-
GCbox CAATbox TATA
box
-
GCCCAATCTTATAAA I.r
GGGCGGO 8AD gene YOJNSNNE3
(00 • 80 25 *UIXOYS 1018IO
413
npstyeam
72-71678 N.7
E*
polyadenuiation
fromnnclear protett mRNA
dearadationna
to move
siqnol® MRNA outof nucleusandinitiatestransiationin cytoplasm
kozaksegnence 21m0Mt 8 IS NY0IN0SYISTTB4N
recoanizestart codon
AVG 2137000
030-40 WNO!
Jong
franscription
No
abg bocosnani
leave
Shine sequencerin
Daigarno xodprokaryotic
19810 x0c
phases: TO.1 Xod
initiation RNA ANMANEUA
bindingofpolUI SANONAJE MASTIAGN:12:1U
ciongation: qrowing nucieotide
chain
termination:hairpin strncture YSONANNS
transcription nnit TU
DAAseanence intoRNA
that is transcribed contains thAtdonotcode
infrons protein
-
splicino removes
intronsMRNA 1N3m319 197OMOMG
gene
seanencea
for thatcodes
continuous
strands
DNA
protein
intDexonso
broken noirAsupnota
NJNSN.R9YI
29001811
-
N:IwSSARAGa8O
30NOECAINAASAO DIBONMI8T
EIGO
noticRNA
NistolgEukay AIOT
ZNOINDO
PolymeraSeSENA MONL
SASO
DATNG3
BURT1n9N9I9nortiROSY
RNApol Il entering DNAgriped 70W bu 319 :
SynthesisMRNA
(downstream) precursorseTatcorn O NTO
290 19040-65R (TIMATAT
(subunts)12
porypeptides
Rpb Rpb 12
DNA
transcribed &2:largest Rpb
mpstream ) cieft formsdeep
SMAn phosphorviated:Rpb14b:
6x 10Ago 0782i transuriptioninvolves
phos phoryiation
mediatorr binds RNACxit bridge 081T,0W04
enzyme
shifts NS
Carboxyl- Rpbhas transiocation
terminal
domain
(CTD)
acti veSite WAII
3N.YN
1h.g
mg*t
enzymatic reaction DNA-
RNAhybrid statekeep
is usedin
MgCla PCR axisat90°
rndder.lid
hybrid meiting
, RNA
pol Il interauts
with
promoters
upstreamrequiatory elements 0077071020873
ONR 3008
SONT3N913
enhancers
09:94712 3/90
promoters
0*03
Y950MOYG 2imomads
joYtmoo lomixoye
enkaryotic promoters
otre (A Regmiatory Region Region core
-
M9YT20
US8.D
X00OTOTI 781J xod X00D
Enhancer UAS LUA'S ATAT IT3R DDl ORFDADED
0 0.
Distal Proximal
(»ikb) (4 Kb)
TSS MASIZON
TanStart- site
30 +1 +30 IN0ANINNSDAN0Q
BREHITATANHONRDPEHAMAMHOTONE
291NITINE
00772I2NSNT DND
2N9UNN NbDfTFLLID
MI0N2.0/g07 #fauntates
binding
3hN9NN9E
URE npstreamreguiatory elements
1ow
altivity of promoters 215 oobm2rA18 9xiN00281
greatlyincreased
SPI box GCbox or
pres
by othen
of ence
elements ofthe
upstream promotera
0ORFI AOST
292 5 NG
CLATbox
UAS activating segmence
npstream ILloq
NO1ERONGIA
owa
telpnibnid R0TBATIMI
aniaNo S0G09I3NNRowYN
UAS +Enhancer
: activatorbinding sites NdNte
nigNiAN:N0lM7c9 L
tiaN n07qlJ2N0YL
MisioU
RSot
Silencermi ZILANE
sitesNENE IONAAASEAMO
--
xepressok
bindinq
2N0TFm
aaNomaY
Onibige Abam
3n3
corepromoter elements R304 4ANT
29000 9309NA3L
MVG
CpGislands
highfreguency ofCG noDd
Q7NDI3N a0.amAwa
aNONMiintaAiGN
silencino islands
ofcpG
methyation isassociated
with
TATA
BRE
60i.Ofprotein
recoonition
lack
Codingqene
TFIß
eiement
obvious AV930:0D
AndinmiSmjo
ILid9 AMA
GlC GlC GAG-AG3L
TATAbox notal genes TATA
contain boxNasnq neieNihNe
AUS
TATA other
(AIT)A(AIT) genes ContainsINR
(aNR: Initiatonog CI prokaryotic Promoter
Lat -Ia.-AattI 2. motifs
have atialddiennit
N--AN-NEAIT
(w) and-35 MA0Y12qV
439D 2N2D
.
nncreobase eukaryotic onin ? have
purimidine iSTATA
-30which box
Ot
2 (clt)dGICAIT)
NO7NIADONGEONG
: downstream
DPE
Pu
exement/
corepromoter
T- G
GEAIT.7C G 9v0d8
1x3-APTOicaiam aAmid:
V 0oltA00I8N51T INadaadcon 0g2
ID0ioist niomod(473
purine
1100 31ial3v80 AM
(GIA
DORASANTDAMNADG:
099x DIDUNAMA-AUAO
.97.012 212A
AJSmbszaLal
2i&A0P*A
l.TONANY
LoniSmbdNd:
, transcriptionPattor
HE
COTAVOTOA
formation
basalof fautor
franscription
Fautors
transcription
tomplex
topromoter
thatbind and initrate
transcription
general
basal
factor
transcription (GTFs) of
types
L 91783
transcriptionmachinery
dst oxe
TSDAANNAN 1 41.000
s80
basal transcription
level of transcription
(atla fromcore
promoter:
TFILE TFIIF 119379.7819/
TFUB
TFIID
TFIIA TFIH
+h MTAST
polll RNA TSS 1/91 813
-
TBP bindinaprotein TATA 1N9MS19
90ISN932
TATAboxbinds to minor of DNA
groove at EODEEP
bOXif promorersdonotcontainTATA
retruited
TBP InR
reernits and
otherDNAbindina
TFs protein
RNA
and polIl
T6Pbinds
atoiS
in similarAJ TATA
19I
manner
as
box
RNArequivedfor all pol 3 ONTc
,
-
1T
RNApolI 5.8 18 288 TRNA
RNA pOI UII 5s rRNA
1n higherenkarmotes ofTBP
theAssociation with bydiftpoliS
transcription
(TBPassociated factor)TAF
determinedbyinteraction
with
TAUD complex AtleastTAFIlsare
associatedwithTBPin theg
TFIID
for
required autivation
trnnseriptronal B No
0ARNO
binds TATA
box major seauence
speuific DNAbinding component
TFIIA TBP TFIID : TAFnS
Stabilizes TFIID 9N 9200080T MNIS 2
anti repressionfunction ANTG
TFII 6 S CIY0029Y 27
NV 1310017ND3
startsite seieution
it8 31713392 101A
TFIIF 1ASNET
TFILE
non specificRNA
destabilize polII-DNAonteraution
N0D YSONONNS
TEILH NANiD N83A0214303
9144onTN a1oSVRT
hydrolysispromoter meitingand
clearancebyATP
phosphorylate
eionaation
RNARpbI at
phasebeqin To
polIl at lTDkinase
CTD activity
repair compiing DNA
siteinitiationat
transcriptionpromoter
pol I1 + pre-initiation complex(PIL)
RNA
GTF
interaction
protein-protein TF(eXcept
Other TFIID. viaenter
TBP) PIC
IN
TRANSCRIPTION
INITIATION
ELKAR
genestructuretranscription
D
8R030NNNTOTANA3N
20JNRIN9
genestructure
2092OMIOA
proximal control elements corepromoter 8703Cl00bYd
6 910 NO1
ASZ
U1C1A site
PbIyCA)
-•
donnstream
-
GCbox CAATbox TATA
box
-
GCCCAATCTTATAAA I.r
GGGCGGO 8AD gene YOJNSNNE3
(00 • 80 25 *UIXOYS 1018IO
413
npstyeam
72-71678 N.7
E*
polyadenuiation
fromnnclear protett mRNA
dearadationna
to move
siqnol® MRNA outof nucleusandinitiatestransiationin cytoplasm
kozaksegnence 21m0Mt 8 IS NY0IN0SYISTTB4N
recoanizestart codon
AVG 2137000
030-40 WNO!
Jong
franscription
No
abg bocosnani
leave
Shine sequencerin
Daigarno xodprokaryotic
19810 x0c
phases: TO.1 Xod
initiation RNA ANMANEUA
bindingofpolUI SANONAJE MASTIAGN:12:1U
ciongation: qrowing nucieotide
chain
termination:hairpin strncture YSONANNS
transcription nnit TU
DAAseanence intoRNA
that is transcribed contains thAtdonotcode
infrons protein
-
splicino removes
intronsMRNA 1N3m319 197OMOMG
gene
seanencea
for thatcodes
continuous
strands
DNA
protein
intDexonso
broken noirAsupnota
NJNSN.R9YI
29001811
-
N:IwSSARAGa8O
30NOECAINAASAO DIBONMI8T
EIGO
noticRNA
NistolgEukay AIOT
ZNOINDO
PolymeraSeSENA MONL
SASO
DATNG3
BURT1n9N9I9nortiROSY
RNApol Il entering DNAgriped 70W bu 319 :
SynthesisMRNA
(downstream) precursorseTatcorn O NTO
290 19040-65R (TIMATAT
(subunts)12
porypeptides
Rpb Rpb 12
DNA
transcribed &2:largest Rpb
mpstream ) cieft formsdeep
SMAn phosphorviated:Rpb14b:
6x 10Ago 0782i transuriptioninvolves
phos phoryiation
mediatorr binds RNACxit bridge 081T,0W04
enzyme
shifts NS
Carboxyl- Rpbhas transiocation
terminal
domain
(CTD)
acti veSite WAII
3N.YN
1h.g
mg*t
enzymatic reaction DNA-
RNAhybrid statekeep
is usedin
MgCla PCR axisat90°
rndder.lid
hybrid meiting
, RNA
pol Il interauts
with
promoters
upstreamrequiatory elements 0077071020873
ONR 3008
SONT3N913
enhancers
09:94712 3/90
promoters
0*03
Y950MOYG 2imomads
joYtmoo lomixoye
enkaryotic promoters
otre (A Regmiatory Region Region core
-
M9YT20
US8.D
X00OTOTI 781J xod X00D
Enhancer UAS LUA'S ATAT IT3R DDl ORFDADED
0 0.
Distal Proximal
(»ikb) (4 Kb)
TSS MASIZON
TanStart- site
30 +1 +30 IN0ANINNSDAN0Q
BREHITATANHONRDPEHAMAMHOTONE
291NITINE
00772I2NSNT DND
2N9UNN NbDfTFLLID
MI0N2.0/g07 #fauntates
binding
3hN9NN9E
URE npstreamreguiatory elements
1ow
altivity of promoters 215 oobm2rA18 9xiN00281
greatlyincreased
SPI box GCbox or
pres
by othen
of ence
elements ofthe
upstream promotera
0ORFI AOST
292 5 NG
CLATbox
UAS activating segmence
npstream ILloq
NO1ERONGIA
owa
telpnibnid R0TBATIMI
aniaNo S0G09I3NNRowYN
UAS +Enhancer
: activatorbinding sites NdNte
nigNiAN:N0lM7c9 L
tiaN n07qlJ2N0YL
MisioU
RSot
Silencermi ZILANE
sitesNENE IONAAASEAMO
--
xepressok
bindinq
2N0TFm
aaNomaY
Onibige Abam
3n3
corepromoter elements R304 4ANT
29000 9309NA3L
MVG
CpGislands
highfreguency ofCG noDd
Q7NDI3N a0.amAwa
aNONMiintaAiGN
silencino islands
ofcpG
methyation isassociated
with
TATA
BRE
60i.Ofprotein
recoonition
lack
Codingqene
TFIß
eiement
obvious AV930:0D
AndinmiSmjo
ILid9 AMA
GlC GlC GAG-AG3L
TATAbox notal genes TATA
contain boxNasnq neieNihNe
AUS
TATA other
(AIT)A(AIT) genes ContainsINR
(aNR: Initiatonog CI prokaryotic Promoter
Lat -Ia.-AattI 2. motifs
have atialddiennit
N--AN-NEAIT
(w) and-35 MA0Y12qV
439D 2N2D
.
nncreobase eukaryotic onin ? have
purimidine iSTATA
-30which box
Ot
2 (clt)dGICAIT)
NO7NIADONGEONG
: downstream
DPE
Pu
exement/
corepromoter
T- G
GEAIT.7C G 9v0d8
1x3-APTOicaiam aAmid:
V 0oltA00I8N51T INadaadcon 0g2
ID0ioist niomod(473
purine
1100 31ial3v80 AM
(GIA
DORASANTDAMNADG:
099x DIDUNAMA-AUAO
.97.012 212A
AJSmbszaLal
2i&A0P*A
l.TONANY
LoniSmbdNd:
, transcriptionPattor
HE
COTAVOTOA
formation
basalof fautor
franscription
Fautors
transcription
tomplex
topromoter
thatbind and initrate
transcription
general
basal
factor
transcription (GTFs) of
types
L 91783
transcriptionmachinery
dst oxe
TSDAANNAN 1 41.000
s80
basal transcription
level of transcription
(atla fromcore
promoter:
TFILE TFIIF 119379.7819/
TFUB
TFIID
TFIIA TFIH
+h MTAST
polll RNA TSS 1/91 813
-
TBP bindinaprotein TATA 1N9MS19
90ISN932
TATAboxbinds to minor of DNA
groove at EODEEP
bOXif promorersdonotcontainTATA
retruited
TBP InR
reernits and
otherDNAbindina
TFs protein
RNA
and polIl
T6Pbinds
atoiS
in similarAJ TATA
19I
manner
as
box
RNArequivedfor all pol 3 ONTc
,
-
1T
RNApolI 5.8 18 288 TRNA
RNA pOI UII 5s rRNA
1n higherenkarmotes ofTBP
theAssociation with bydiftpoliS
transcription
(TBPassociated factor)TAF
determinedbyinteraction
with
TAUD complex AtleastTAFIlsare
associatedwithTBPin theg
TFIID
for
required autivation
trnnseriptronal B No
0ARNO
binds TATA
box major seauence
speuific DNAbinding component
TFIIA TBP TFIID : TAFnS
Stabilizes TFIID 9N 9200080T MNIS 2
anti repressionfunction ANTG
TFII 6 S CIY0029Y 27
NV 1310017ND3
startsite seieution
it8 31713392 101A
TFIIF 1ASNET
TFILE
non specificRNA
destabilize polII-DNAonteraution
N0D YSONONNS
TEILH NANiD N83A0214303
9144onTN a1oSVRT
hydrolysispromoter meitingand
clearancebyATP
phosphorylate
eionaation
RNARpbI at
phasebeqin To
polIl at lTDkinase
CTD activity
repair compiing DNA
siteinitiationat
transcriptionpromoter
pol I1 + pre-initiation complex(PIL)
RNA
GTF
interaction
protein-protein TF(eXcept
Other TFIID. viaenter
TBP) PIC
