MICROBIOLOGY FINAL- LAB UPDATED ACTUAL EXAM
QUESTIONS AND CORRECT ANSWERS
Question 1.
Lab 1
ANSWER
Question 2.
What three elements are used in an autoclave to sterilize equipment?
ANSWER
heat, pressure, and steam
Question 3.
What is the minimum temperature an autoclave must be set at to achieve sterile condition?
ANSWER
125°C
Question 4.
What type of incubator is pictured below?
ANSWER
Fixed incubator
,Question 5.
If you are working in a lab in which an autoclave is not available, and you are pressed for time, which
would you choose to best sterilize your equipment? Hot steam or hot air? Explain why you chose
your answer.
ANSWER
Hot steam is the best choice as you can achieve a sterile environment in a matter of minutes
whereas hot air will take several hours to achieve the same effect.
Question 6.
What are the main sections that should be found in a lab notebook? Name at least 4.
ANSWER
Objective, Procedure, Notes, Results and Deviations
Question 7.
At what temperature should you refrigerate bacterial samples? Explain why this is beneficial.
ANSWER
4°C. This temperature slows bacterial growth and prolongs the life of the sample.
Question 8.
What are the FOUR types of gloves presented in the lab video?
ANSWER
, Latex, Nitrile, Thermal cold, Thermal heat
Question 9.
At what temperature is the fixed incubator set to, as presented in the lab video?
ANSWER
37°C
Question 10.
What THREE rules were discussed in regards to lab safety that would protect you and others from
contamination?
ANSWER
1. Never eat or drink in the lab2. Always wear appropriate PPE3. Never leave the lab wearing
PPE
Question 11.
You are a lab instructor, and Sally Miller has turned in her lab notebook for you to grade the lab
experiment #1 on lab safety. Based upon what was covered in the lab video, how should Sally have
titled her experiment?
ANSWER
SM01 Lab Safety
Question 12.
You arrive to your first day of work at a new lab. You are taking over for someone who took a new
job at another lab. Your boss informs you that because of time restraints, this person did not exactly
follow the experimental protocol. In order to proceed, you must know what he did differently. (1)
According to the lab lecture, under what section of his notebook would you look to find the
experimental steps? (2) As changes to the experimental steps were made, what are these
differences called, and how should it appear in the lab notebook?
ANSWER
(1) Procedure—this is where the steps for the experimental protocol are recorded.(2)
Deviations. All deviations should be written in red to immediately bring attention to the
changes in the protocol.
QUESTIONS AND CORRECT ANSWERS
Question 1.
Lab 1
ANSWER
Question 2.
What three elements are used in an autoclave to sterilize equipment?
ANSWER
heat, pressure, and steam
Question 3.
What is the minimum temperature an autoclave must be set at to achieve sterile condition?
ANSWER
125°C
Question 4.
What type of incubator is pictured below?
ANSWER
Fixed incubator
,Question 5.
If you are working in a lab in which an autoclave is not available, and you are pressed for time, which
would you choose to best sterilize your equipment? Hot steam or hot air? Explain why you chose
your answer.
ANSWER
Hot steam is the best choice as you can achieve a sterile environment in a matter of minutes
whereas hot air will take several hours to achieve the same effect.
Question 6.
What are the main sections that should be found in a lab notebook? Name at least 4.
ANSWER
Objective, Procedure, Notes, Results and Deviations
Question 7.
At what temperature should you refrigerate bacterial samples? Explain why this is beneficial.
ANSWER
4°C. This temperature slows bacterial growth and prolongs the life of the sample.
Question 8.
What are the FOUR types of gloves presented in the lab video?
ANSWER
, Latex, Nitrile, Thermal cold, Thermal heat
Question 9.
At what temperature is the fixed incubator set to, as presented in the lab video?
ANSWER
37°C
Question 10.
What THREE rules were discussed in regards to lab safety that would protect you and others from
contamination?
ANSWER
1. Never eat or drink in the lab2. Always wear appropriate PPE3. Never leave the lab wearing
PPE
Question 11.
You are a lab instructor, and Sally Miller has turned in her lab notebook for you to grade the lab
experiment #1 on lab safety. Based upon what was covered in the lab video, how should Sally have
titled her experiment?
ANSWER
SM01 Lab Safety
Question 12.
You arrive to your first day of work at a new lab. You are taking over for someone who took a new
job at another lab. Your boss informs you that because of time restraints, this person did not exactly
follow the experimental protocol. In order to proceed, you must know what he did differently. (1)
According to the lab lecture, under what section of his notebook would you look to find the
experimental steps? (2) As changes to the experimental steps were made, what are these
differences called, and how should it appear in the lab notebook?
ANSWER
(1) Procedure—this is where the steps for the experimental protocol are recorded.(2)
Deviations. All deviations should be written in red to immediately bring attention to the
changes in the protocol.
