BIOD 171 MODULE 3 EXAM
COMPREHENSIVE QUESTIONS AND
SOLUTIONS
◉ microscopy
Answer: the use of light or electrons to magnify objects
◉ Micrometer (um)
Answer: 10^-6 m, measures size of cells
◉ Nanometer (nm)
Answer: 10^-9m, used to measure cellular organelles and viruses
◉ Magnification
Answer: the ratio of an object's image size to its real size
◉ Resolution of a microscope
Answer: ability to distinguish two objects as separate entities
◉ Contrast Microscopy
Answer: shows presence of dye or other treatments
, ◉ Scanning Tunneling Microscope (STM)
Answer: microscope that measures electrons that leak or "tunnel"
from the surface of the specimen
0.01nm-10nm can see atoms amino acids DNA and proteins
◉ Transmission Electron Microscope (TEM)
Answer: 0.078-100um can see atoms, DNA, amino, proteins,
ribosomes, viruses, bacteria and archaea, mitochondria,
chloroplasts, human blood cells
◉ scanning electron microscope
Answer: 0.4-1mm amino acids - red blood cells
◉ compound light microscope
Answer: 200 nm - 10 mm can see bacteria and archaea,
organelles, blood cells, and large protozoa
◉ light microscope
Answer: use visible light and blue wavelengths for improved
resolution, magnification 1x-2000x resolution: 10nm-200nm can
see whole cells and organelles
◉ electron microscope
COMPREHENSIVE QUESTIONS AND
SOLUTIONS
◉ microscopy
Answer: the use of light or electrons to magnify objects
◉ Micrometer (um)
Answer: 10^-6 m, measures size of cells
◉ Nanometer (nm)
Answer: 10^-9m, used to measure cellular organelles and viruses
◉ Magnification
Answer: the ratio of an object's image size to its real size
◉ Resolution of a microscope
Answer: ability to distinguish two objects as separate entities
◉ Contrast Microscopy
Answer: shows presence of dye or other treatments
, ◉ Scanning Tunneling Microscope (STM)
Answer: microscope that measures electrons that leak or "tunnel"
from the surface of the specimen
0.01nm-10nm can see atoms amino acids DNA and proteins
◉ Transmission Electron Microscope (TEM)
Answer: 0.078-100um can see atoms, DNA, amino, proteins,
ribosomes, viruses, bacteria and archaea, mitochondria,
chloroplasts, human blood cells
◉ scanning electron microscope
Answer: 0.4-1mm amino acids - red blood cells
◉ compound light microscope
Answer: 200 nm - 10 mm can see bacteria and archaea,
organelles, blood cells, and large protozoa
◉ light microscope
Answer: use visible light and blue wavelengths for improved
resolution, magnification 1x-2000x resolution: 10nm-200nm can
see whole cells and organelles
◉ electron microscope