QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS lOMoARcPSD| 69811638
UAMS MICROBIOLOGY FINAL
QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS GUARANTEED
SUCCESS!!!
Microbiology: Gram-Positive Cocci Identification Matrix
Which organism is identified by a Positive (Black) Bile Esculin Agar
(BEA) test, Growth in 6.5% Salt, and Optochin Resistance?
✔✔ Enterococcus species
Which organism is identified by a Positive (Black) Bile Esculin Agar
(BEA) test, No Growth in 6.5% Salt, and Optochin Resistance?
✔✔ Group D Streptococcus (non-enterococcus, e.g., Streptococcus bovis /
Streptococcus gallolyticus)
Which organism is identified by a Colorless (Negative) Bile Esculin Agar
(BEA) test, No Growth in 6.5% Salt, and Optochin Susceptibility?
✔✔ Streptococcus pneumoniae
Which organism is identified by a Colorless (Negative) Bile Esculin Agar
(BEA) test, No Growth in 6.5% Salt, and Optochin Resistance?
✔✔ Viridans Group Streptococci
Hemolysis: Beta
Gram Stain: GP Coccobacilli
Catalase: +
BEA: +
CAMP/Hippurate: +
Motility: + @ 25 C -ANSWER✔✔Listeria
What organisms are motile at 25 C? -ANSWER✔✔Yersinia entercolitica
& Listeria
What GPR organisms form spores? -ANSWER✔✔Bacillus species
(aerobic)
Clostridium species (anaerobic)
)
, QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS lOMoARcPSD| 69811638
What temperature should CIN agar be incubated at? -ANSWER✔✔RT
Selective for Gram-Negative Bacilli -ANSWER✔✔EMB & MAC
Selective media for Gram-positive organisms -ANSWER✔✔CNA & PEA
Gram staining steps -ANSWER✔✔1. crystal violet
2. iodine
3. alcohol
4. safranin
ID Of Staphylococci -ANSWER✔✔-Slide coagulase
-Tube coagulase
-MSA
-Latex agglutination
-Novobiocin
Reagent: Bile Solubility -ANSWER✔✔10% deoxycholate (bile)
Enhance growth of specific MOs, while inhibiting the growth of others
(GN & Selenite) -ANSWER✔✔Enrichment Broths
No antimicrobial substances & allows growth of most organisms (BAP,
CHOC) -ANSWER✔✔Nonselective Media
Contains antimicrobial substances, allows growth of specific bacteria
while inhibiting the growth of others (PEA, CNA) -
ANSWER✔✔Selective Media
Basic growth media for bacteria (BAP/ CHOC) -ANSWER✔✔Nutrient
Media
Metabolic differences and biochemical reactions in the media
distinguishes MO (ph Color indicator) -ANSWER✔✔Differential Media
)
, QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS lOMoARcPSD| 69811638
Reagent: Catalase -ANSWER✔✔Hydrogen Peroxide
Reagent: Coagulase -ANSWER✔✔Plasma
What is CHROMagar used for? -ANSWER✔✔MRSA
Sticky Staph
Cat: =
Oxidase: =
Bacitracin: R -ANSWER✔✔Rothia
Reagent: PYR -ANSWER✔✔N,N dimethylaminocinnamaldehyde
What causes satelliting Strep? -ANSWER✔✔Deficiency in Vitamin B6
(pyridoxal)
Gamma Hemolysis Strep Tests -ANSWER✔✔-BEA
-Salt
-PYR
-CAMP/ Hipp
Alpha Hemolysis Strep Tests -ANSWER✔✔-BEA
-Salt
-P Disk
-PYR
Beta Hemolysis Strep Tests -ANSWER✔✔-A Disk
-SXT
-PYR
-CAMP/Hipp
What is the hemolysis for Group C, F & G Strep? -ANSWER✔✔Beta
)
UAMS MICROBIOLOGY FINAL
QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS GUARANTEED
SUCCESS!!!
Microbiology: Gram-Positive Cocci Identification Matrix
Which organism is identified by a Positive (Black) Bile Esculin Agar
(BEA) test, Growth in 6.5% Salt, and Optochin Resistance?
✔✔ Enterococcus species
Which organism is identified by a Positive (Black) Bile Esculin Agar
(BEA) test, No Growth in 6.5% Salt, and Optochin Resistance?
✔✔ Group D Streptococcus (non-enterococcus, e.g., Streptococcus bovis /
Streptococcus gallolyticus)
Which organism is identified by a Colorless (Negative) Bile Esculin Agar
(BEA) test, No Growth in 6.5% Salt, and Optochin Susceptibility?
✔✔ Streptococcus pneumoniae
Which organism is identified by a Colorless (Negative) Bile Esculin Agar
(BEA) test, No Growth in 6.5% Salt, and Optochin Resistance?
✔✔ Viridans Group Streptococci
Hemolysis: Beta
Gram Stain: GP Coccobacilli
Catalase: +
BEA: +
CAMP/Hippurate: +
Motility: + @ 25 C -ANSWER✔✔Listeria
What organisms are motile at 25 C? -ANSWER✔✔Yersinia entercolitica
& Listeria
What GPR organisms form spores? -ANSWER✔✔Bacillus species
(aerobic)
Clostridium species (anaerobic)
)
, QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS lOMoARcPSD| 69811638
What temperature should CIN agar be incubated at? -ANSWER✔✔RT
Selective for Gram-Negative Bacilli -ANSWER✔✔EMB & MAC
Selective media for Gram-positive organisms -ANSWER✔✔CNA & PEA
Gram staining steps -ANSWER✔✔1. crystal violet
2. iodine
3. alcohol
4. safranin
ID Of Staphylococci -ANSWER✔✔-Slide coagulase
-Tube coagulase
-MSA
-Latex agglutination
-Novobiocin
Reagent: Bile Solubility -ANSWER✔✔10% deoxycholate (bile)
Enhance growth of specific MOs, while inhibiting the growth of others
(GN & Selenite) -ANSWER✔✔Enrichment Broths
No antimicrobial substances & allows growth of most organisms (BAP,
CHOC) -ANSWER✔✔Nonselective Media
Contains antimicrobial substances, allows growth of specific bacteria
while inhibiting the growth of others (PEA, CNA) -
ANSWER✔✔Selective Media
Basic growth media for bacteria (BAP/ CHOC) -ANSWER✔✔Nutrient
Media
Metabolic differences and biochemical reactions in the media
distinguishes MO (ph Color indicator) -ANSWER✔✔Differential Media
)
, QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS lOMoARcPSD| 69811638
Reagent: Catalase -ANSWER✔✔Hydrogen Peroxide
Reagent: Coagulase -ANSWER✔✔Plasma
What is CHROMagar used for? -ANSWER✔✔MRSA
Sticky Staph
Cat: =
Oxidase: =
Bacitracin: R -ANSWER✔✔Rothia
Reagent: PYR -ANSWER✔✔N,N dimethylaminocinnamaldehyde
What causes satelliting Strep? -ANSWER✔✔Deficiency in Vitamin B6
(pyridoxal)
Gamma Hemolysis Strep Tests -ANSWER✔✔-BEA
-Salt
-PYR
-CAMP/ Hipp
Alpha Hemolysis Strep Tests -ANSWER✔✔-BEA
-Salt
-P Disk
-PYR
Beta Hemolysis Strep Tests -ANSWER✔✔-A Disk
-SXT
-PYR
-CAMP/Hipp
What is the hemolysis for Group C, F & G Strep? -ANSWER✔✔Beta
)
