SUCCESS! IN CLINICAL LABORATORY
SCIENCE- IMMUNOHEMATOLOGY EXAM
1. A woman wants to donate blood. Her physical examination reveals the following:
weight—110 lbs, pulse—73 bpm, blood pressure—125/75 mm Hg, hematocrit—35%.
Which of the following exclusions applies to the prospective donor?
A. Pulse too high
B. Weight too low
C. Hematocrit too low
D. Blood pressure too low - Correct Answers -C. Hematocrit too low
2. A potential donor has no exclusions, but she weighs only 87 pounds. What is the
allowable amount of blood (including samples) that can be drawn?
A. 367 mL
B. 378 mL
C. 415 mL
D. 473 mL - Correct Answers -C. 415 mL
3. A donor at a blood drive becomes sweaty and agitated during the donation. She
appears pale and limp. What is the best course of action for this situation?
A. Continue the donation
B. Withdraw the needle, lift her feet, and administer ammonia
C. Discontinue donation and provide a paper bag
D. Call ambulanc - Correct Answers -B. Withdraw the needle, lift her feet, and
administer ammonia
4. How much anticoagulant would need to be removed from a 450 mL blood bag if a
donor weighed 94 pounds?
A. 9 mL
B. 10 mL
C. 12 mL
D. 15 mL - Correct Answers -A. 9 mL
5. Which of the following donors would be deferred indefinitely?
A. History of syphilis
B. History of gonorrhea
C. Accutane® treatment
D. Recipient of human growth hormone - Correct Answers -D. Recipient of human
growth hormone
6. Which of the following viruses resides exclusively in leukocytes?
,A. Cytomegalovirus (CMV)
B. Human immunodeficient virus (HIV)
C. Hepatitis B virus (HBV)
D. Hepatitis C virus (HCV) - Correct Answers -A. Cytomegalovirus (CMV)
7. A 47-year-old women donated a unit of blood at a church blood drive. She meets all
the physical criteria to do-nate, but she takes a blood thinner and B12 injections. Is she
an acceptable donor?
A. No, due to blood thinner
B. No, due to B12 injections
C. Yes, for platelets only
D. Yes, red cells only - Correct Answers -D. Yes, red cells only
8. Which of the following best describes what must be done with a unit of blood drawn
from a donor who is found to be at high risk of contracting acquired immune deficiency
syndrome (AIDS)?
A. Hold unit in quarantine until donor diagnosis is clarified.
B. Use the blood for research dealing with AIDS.
C. Properly dispose of unit by autoclaving or incineration.
D. Use the plasma and destroy the red blood cells. - Correct Answers -C. Properly
dispose of unit by autoclaving or incineration.
9. Which of the following is least likely to transmit hepatitis?
A. Cryoprecipitate
B. RBC
C. Plasma protein fraction (PPF)
D. Platelets - Correct Answers -C. Plasma protein fraction (PPF)
10. A pooled serum product from 16 donors has a repeatedly positive nucleic acid test
(NAT) for HCV. The next action that should be taken is to:
A. permanently exclude all the donors in the pool.
B. test each donor in the pool for HCV.
C. label all the donors as HCV positive.
D. confirm the positive using a recombi-nant immunoblot assay. - Correct Answers -B.
test each donor in the pool for HCV.
11. Although cryoprecipitate has primarily been used for treatment of
hypofibrinogenemia and hemophilia A, it contains other blood proteins useful in the
treatment of coagu-lopathies. Which of the following is not found in cryoprecipitate?
A. Fibronectin
B. Factor XIII
C. Factor VIII:vW
D. Antithrombin III - Correct Answers -D. Antithrombin III
12. Even though it is properly collected and stored, which of the following will fresh-
frozen plasma (FFP) not provide?
,A. Factor V
B. Factor VIII
C. Factor IX
D. Platelets - Correct Answers -D. Platelets
13. Blood needs to be prepared for intrauterine transfusion of a fetus with severe
hemolytic disease of the newborn (HDN). The red blood cell unit selected is compatible
with the mother's serum and has been leuko-depleted. An additional step that must be
taken before transfusion is to:
A. add pooled platelets and fresh-frozen plasma.
B. check that the RBC group is consistent with the father's.
C. irradiate the RBCs before infusion.
D. test the RBC unit with the neonate's eluate - Correct Answers -C. irradiate the RBCs
before infusion.
14. The addition of adenine in an anticoagulant-preservative formulation aids in which of
the following?
A. Maintaining ATP levels for red cell vi-ability B. Maintaining platelet function in stored
blood C. Reducing the plasma K+ levels during storage
D. Maintaining 2,3-BPG levels for oxygen release to the tissues - Correct Answers -A.
Maintaining ATP levels for red cell vi-ability B. Maintaining
15. The pilot tubes for donor unit #3276 break in the centrifuge. What should be done to
resolve this issue?
A. Label the blood using the donor's previ-ous records.
B. Discard the unit because processing procedures cannot be performed.
C. Discard the RBCs and salvage the plasma for fractionation.
D. Remove sufficient segments to complete donor processing procedures - Correct
Answers -D. Remove sufficient segments to complete donor processing procedures
16. A satellite bag containing 250 mL of fresh plasma is selected for quality control of
cryoprecipitate production. Cryoprecipitate is prepared according to standard operat-ing
procedures. The final product has a total volume of 10 mL. The factor VIII assays are 1
IU/mL before and 9 IU/mL after preparation.
What is the percent yield of factor VIII in the final cryoprecipitate?
A. 11%
B. 25%
C. 36%
D. 80 - Correct Answers -C. 36%
17. A satellite bag containing 250 mL of fresh plasma is selected for quality control of
cryoprecipitate production. Cryoprecipitate is prepared according to standard operat-ing
procedures. The final product has a total volume of 10 mL. The factor VIII assays are 1
IU/mL before and 9 IU/mL after preparation
Does this product meet AABB Standards for cryoprecipitate production?
A. Yes
, B. No; the percent recovery is too low.
C. No; the final factor VIII level is too low.
D. Data are insufficient to calculate - Correct Answers -A. Yes
18. What should be done with a unit of RBCs that was irradiated twice?
A. Change expiration date and then issue the unit.
B. Note on unit that it has been irradiated twice.
C. Discard the unit.
D. Issue immediately. - Correct Answers -C. Discard the unit.
19. What is the expiration date/time of pooled cryoprecipitate?
A. 30 minutes
B. 2 hours
C. 4 hours
D. 8 hours - Correct Answers -C. 4 hours
20. Which of the following is most accurate regarding platelet apheresis criteria?
A. The minimum platelet count must be 3.0 * 1011, pH must be Ú6.0.
B. The minimum platelet count must be 3.0 * 1010, pH must be ...6.2.
C. The minimum platelet count must be 3.0 * 1011, pH must be Ú6.2.
D. The minimum platelet count must be 5.5 * 1010, pH must be ...6.0. - Correct Answers
-C. The minimum platelet count must be 3.0 * 1011, pH must be Ú6.2.
21. Which of the following lists the correct shelf life?
A. Frozen RBCs at -65°C = 7 years
B. Fresh frozen plasma (FFP) at -18°C = 1 year
C. Fresh frozen plasma (FFP) at -65°C = 1 year D. Platelets at 6°C = 5 day - Correct
Answers -B. Fresh frozen plasma (FFP) at -18°C = 1 year
22. When 2,3-BPG levels drop in stored blood, which of the following occurs as a
result?
A. RBC K+ increases.
B. RBC ability to release O2 decreases.
C. Plasma hemoglobin is stabilized.
D. ATP synthesis increases. - Correct Answers -B. RBC ability to release O2
decreases.
23. The last unit of autologous blood for an elective surgery patient should be collected
no later than ________________ hours before surgery. A. 24
B. 36
C. 48
D. 72 - Correct Answers -D. 72
24. For which of the following patients would autologous donation not be advisable?
A. Patients with an antibody against a high-incidence antigen
B. Patients with uncompensated anemia
SCIENCE- IMMUNOHEMATOLOGY EXAM
1. A woman wants to donate blood. Her physical examination reveals the following:
weight—110 lbs, pulse—73 bpm, blood pressure—125/75 mm Hg, hematocrit—35%.
Which of the following exclusions applies to the prospective donor?
A. Pulse too high
B. Weight too low
C. Hematocrit too low
D. Blood pressure too low - Correct Answers -C. Hematocrit too low
2. A potential donor has no exclusions, but she weighs only 87 pounds. What is the
allowable amount of blood (including samples) that can be drawn?
A. 367 mL
B. 378 mL
C. 415 mL
D. 473 mL - Correct Answers -C. 415 mL
3. A donor at a blood drive becomes sweaty and agitated during the donation. She
appears pale and limp. What is the best course of action for this situation?
A. Continue the donation
B. Withdraw the needle, lift her feet, and administer ammonia
C. Discontinue donation and provide a paper bag
D. Call ambulanc - Correct Answers -B. Withdraw the needle, lift her feet, and
administer ammonia
4. How much anticoagulant would need to be removed from a 450 mL blood bag if a
donor weighed 94 pounds?
A. 9 mL
B. 10 mL
C. 12 mL
D. 15 mL - Correct Answers -A. 9 mL
5. Which of the following donors would be deferred indefinitely?
A. History of syphilis
B. History of gonorrhea
C. Accutane® treatment
D. Recipient of human growth hormone - Correct Answers -D. Recipient of human
growth hormone
6. Which of the following viruses resides exclusively in leukocytes?
,A. Cytomegalovirus (CMV)
B. Human immunodeficient virus (HIV)
C. Hepatitis B virus (HBV)
D. Hepatitis C virus (HCV) - Correct Answers -A. Cytomegalovirus (CMV)
7. A 47-year-old women donated a unit of blood at a church blood drive. She meets all
the physical criteria to do-nate, but she takes a blood thinner and B12 injections. Is she
an acceptable donor?
A. No, due to blood thinner
B. No, due to B12 injections
C. Yes, for platelets only
D. Yes, red cells only - Correct Answers -D. Yes, red cells only
8. Which of the following best describes what must be done with a unit of blood drawn
from a donor who is found to be at high risk of contracting acquired immune deficiency
syndrome (AIDS)?
A. Hold unit in quarantine until donor diagnosis is clarified.
B. Use the blood for research dealing with AIDS.
C. Properly dispose of unit by autoclaving or incineration.
D. Use the plasma and destroy the red blood cells. - Correct Answers -C. Properly
dispose of unit by autoclaving or incineration.
9. Which of the following is least likely to transmit hepatitis?
A. Cryoprecipitate
B. RBC
C. Plasma protein fraction (PPF)
D. Platelets - Correct Answers -C. Plasma protein fraction (PPF)
10. A pooled serum product from 16 donors has a repeatedly positive nucleic acid test
(NAT) for HCV. The next action that should be taken is to:
A. permanently exclude all the donors in the pool.
B. test each donor in the pool for HCV.
C. label all the donors as HCV positive.
D. confirm the positive using a recombi-nant immunoblot assay. - Correct Answers -B.
test each donor in the pool for HCV.
11. Although cryoprecipitate has primarily been used for treatment of
hypofibrinogenemia and hemophilia A, it contains other blood proteins useful in the
treatment of coagu-lopathies. Which of the following is not found in cryoprecipitate?
A. Fibronectin
B. Factor XIII
C. Factor VIII:vW
D. Antithrombin III - Correct Answers -D. Antithrombin III
12. Even though it is properly collected and stored, which of the following will fresh-
frozen plasma (FFP) not provide?
,A. Factor V
B. Factor VIII
C. Factor IX
D. Platelets - Correct Answers -D. Platelets
13. Blood needs to be prepared for intrauterine transfusion of a fetus with severe
hemolytic disease of the newborn (HDN). The red blood cell unit selected is compatible
with the mother's serum and has been leuko-depleted. An additional step that must be
taken before transfusion is to:
A. add pooled platelets and fresh-frozen plasma.
B. check that the RBC group is consistent with the father's.
C. irradiate the RBCs before infusion.
D. test the RBC unit with the neonate's eluate - Correct Answers -C. irradiate the RBCs
before infusion.
14. The addition of adenine in an anticoagulant-preservative formulation aids in which of
the following?
A. Maintaining ATP levels for red cell vi-ability B. Maintaining platelet function in stored
blood C. Reducing the plasma K+ levels during storage
D. Maintaining 2,3-BPG levels for oxygen release to the tissues - Correct Answers -A.
Maintaining ATP levels for red cell vi-ability B. Maintaining
15. The pilot tubes for donor unit #3276 break in the centrifuge. What should be done to
resolve this issue?
A. Label the blood using the donor's previ-ous records.
B. Discard the unit because processing procedures cannot be performed.
C. Discard the RBCs and salvage the plasma for fractionation.
D. Remove sufficient segments to complete donor processing procedures - Correct
Answers -D. Remove sufficient segments to complete donor processing procedures
16. A satellite bag containing 250 mL of fresh plasma is selected for quality control of
cryoprecipitate production. Cryoprecipitate is prepared according to standard operat-ing
procedures. The final product has a total volume of 10 mL. The factor VIII assays are 1
IU/mL before and 9 IU/mL after preparation.
What is the percent yield of factor VIII in the final cryoprecipitate?
A. 11%
B. 25%
C. 36%
D. 80 - Correct Answers -C. 36%
17. A satellite bag containing 250 mL of fresh plasma is selected for quality control of
cryoprecipitate production. Cryoprecipitate is prepared according to standard operat-ing
procedures. The final product has a total volume of 10 mL. The factor VIII assays are 1
IU/mL before and 9 IU/mL after preparation
Does this product meet AABB Standards for cryoprecipitate production?
A. Yes
, B. No; the percent recovery is too low.
C. No; the final factor VIII level is too low.
D. Data are insufficient to calculate - Correct Answers -A. Yes
18. What should be done with a unit of RBCs that was irradiated twice?
A. Change expiration date and then issue the unit.
B. Note on unit that it has been irradiated twice.
C. Discard the unit.
D. Issue immediately. - Correct Answers -C. Discard the unit.
19. What is the expiration date/time of pooled cryoprecipitate?
A. 30 minutes
B. 2 hours
C. 4 hours
D. 8 hours - Correct Answers -C. 4 hours
20. Which of the following is most accurate regarding platelet apheresis criteria?
A. The minimum platelet count must be 3.0 * 1011, pH must be Ú6.0.
B. The minimum platelet count must be 3.0 * 1010, pH must be ...6.2.
C. The minimum platelet count must be 3.0 * 1011, pH must be Ú6.2.
D. The minimum platelet count must be 5.5 * 1010, pH must be ...6.0. - Correct Answers
-C. The minimum platelet count must be 3.0 * 1011, pH must be Ú6.2.
21. Which of the following lists the correct shelf life?
A. Frozen RBCs at -65°C = 7 years
B. Fresh frozen plasma (FFP) at -18°C = 1 year
C. Fresh frozen plasma (FFP) at -65°C = 1 year D. Platelets at 6°C = 5 day - Correct
Answers -B. Fresh frozen plasma (FFP) at -18°C = 1 year
22. When 2,3-BPG levels drop in stored blood, which of the following occurs as a
result?
A. RBC K+ increases.
B. RBC ability to release O2 decreases.
C. Plasma hemoglobin is stabilized.
D. ATP synthesis increases. - Correct Answers -B. RBC ability to release O2
decreases.
23. The last unit of autologous blood for an elective surgery patient should be collected
no later than ________________ hours before surgery. A. 24
B. 36
C. 48
D. 72 - Correct Answers -D. 72
24. For which of the following patients would autologous donation not be advisable?
A. Patients with an antibody against a high-incidence antigen
B. Patients with uncompensated anemia
