MLS -IMMUNOHEMATOLOGY EXAM
QUESTIONS AND ANSWERS
Minimum hemoglobin concentration in a finger stick from a male donor is? - Correct
Answers -12.5 g/dL (125 g/L)
What is a cause for permanent deferral of blood donation? - Correct Answers -history of
jaundice of an unknown etiology
True or False: Can you accept a blood donor with a temperature of 99 degrees F or
37.3 degrees C? The donor claims he feels well. - Correct Answers -True, yes you can!
The body temp does not exceed 37.5 degrees C.
True or False: Hepatitis B is cause for permanent deferral? - Correct Answers -True,
hepatitis or any liver condition is cause for permanent deferral.
What is the minimum age for blood donation? - Correct Answers -16 (but it also varies
by state), Go with 16.
What is the minimum Hct for blood donation? - Correct Answers -38 Hct.
What is the maximum body temperature a person can have and still donate blood? -
Correct Answers -99.5 degrees F or 37.5 degrees C
What is the maximum range of blood pressure that a person is still able to donate
blood? - Correct Answers -May not exceed 180/100 (S/D)
True or false: Vaccines from dead microbial sources are cause for deferral for blood
donation? - Correct Answers -False. People who have synthetic derivatives or biological
toxoids or dead pathogens are allowed to donate.
True or false: A woman who had a spontaneous abortion 2 months prior is allowed to
donate blood? - Correct Answers -True. This female patient is allowed to donate blood.
Prior to donating blood, what compound should you use to prepare the venous puncture
site? - Correct Answers -Iodine compounds
What is the minimum PLT count to donate PLTs? - Correct Answers -150 x 10^3 (150 x
10^9/L)
What serologic test must be performed on all blood donations? - Correct Answers -
Serologic Test for syphilis
,In PLT preparation from a band-new donor unit, do you warm the unit or cool the unit
during PLT concentration, (extraction). - Correct Answers -You cool the unit to ~ 20-24
degrees C.
What is the most common cause for post transfusion hepatitis? - Correct Answers -
Hepatitis B, HBs Ag.
What laboratory molecular method do we use to confirm HIV in a patient? - Correct
Answers -Western Blot using patient HIV anti-HIV-1 antibodies from his or her serum.
What is the total test used to detect HIV in a patient? - Correct Answers -Serologic tests
for antibodies against HIV and 1 and 2
What is screening test used for HIV detection? - Correct Answers -ELISA test for HIV.
Rejuvenation of a unit of RBCs does what to the donor unit? - Correct Answers -It
restores the 2,3 DPG and ATP to normal levels
True or False: Splitting of a donor RBC unit under sterile and closed conditions, will
cause the donor unit to hold its original expiration date? - Correct Answers -True.
Splitting of a unit under sterile and closed conditions won't breach the unit. The
expiration date is held as original.
A donor unit is split for a pediatric patient into multiple bags. A pediatric quad set was
used. If the original donor unit had an expiration date 35 days from now, what will the
new expiration of peds bags and donor unit be? - Correct Answers -Both will have an
expiration of 35 days from now. We used a closed sterilized device to transfer. In total,
the original expiration of the bag holds true!
A donor unit was split using a closed system at 0800 and has an expiration date of
12/01/2015. Today's date is 11/06/2015. What is the expiration of the split unit? -
Correct Answers -12/01/2015. The same date because the donor unit wasn't breached
and remains sterile.
What is the optimum temperature for frozen RBCs? Why? - Correct Answers --80
degrees C. You're in a cryostat which causes amphorpic crystals to form, water to be
removed in a highly regulated manner, which in total does not lyse all the cells.
A donor unit is issued to the cardiac unit at 0900. The unit is returned at 0910. The unit
was not breached or used. What is the best course of action and why? - Correct
Answers -Put the donor unit back on the shelf in the RBC fridge. It's not breached, still
sterile. It's expiration date holds true as the original outgoing unit.
What is the optimum temperature for storing RBC donor units? - Correct Answers -4
degrees C
, A RBC donor unit was slit in an open system using graduated cylinders at 0934. The
expiration date on the unit is 12/01/2015. Today is 11/06/2015. What is the expiration
date of the new unit? Give me the date. - Correct Answers -The expiration date is 24 hrs
post preparation of a breached unit. The new expiration date is 0934 11/07/2015.
What is the optimum storage temp for cryo AHF? - Correct Answers --20 degrees C.
What is the minimum storage temp for cyro AHF? - Correct Answers --18 degrees C.
After thawing cryo AHF. How much time do you have to transfuse the patient? - Correct
Answers -4 hours. If you go over 4 hours you have to waste the product.
What is the shelf life for cyro AHF in a frozen state? - Correct Answers -12 month / 1
year
After having thawed FFP, how much time do you have to infuse the patient with? -
Correct Answers -24 hours
Irradiation of donor RBC prevents replication of what WBC type? - Correct Answers -
Lymphocytes. All HTRs are correlated through CD4 Th cells, a lymphocyte. If you kill it.
You will have NO response.
What is an important determinant of PLT viability following storage? - Correct Answers -
plasma Ph
Patients who demonstrate bombay blood phenotype have what antibody present in his
or her blood? - Correct Answers -Anti-H anti-body
What antibodies is neutralized by pooled human plasma? why? - Correct Answers -anti-
Ch
anti-Ch and anti-Rg react at IAT (immediate spin, cool phase) and reacts with C4
(component of complement).
What is the minimum pH that platelets can go to before they are considered expired? -
Correct Answers -plasma pH of 6.2 A pH greater than 6.2 has to be maintained at all
times.
In the liquid state, plasma is stored at what temperature? - Correct Answers -1-6
degrees C
Why do we have to irradiate a PLT product from a mother who is doing a donation to
her child? - Correct Answers -The mother's HLA are similar to the child, but not similar
enough. When she donates to the child, because of the lack of similarity; the child would
end up with a type I allergic response.
QUESTIONS AND ANSWERS
Minimum hemoglobin concentration in a finger stick from a male donor is? - Correct
Answers -12.5 g/dL (125 g/L)
What is a cause for permanent deferral of blood donation? - Correct Answers -history of
jaundice of an unknown etiology
True or False: Can you accept a blood donor with a temperature of 99 degrees F or
37.3 degrees C? The donor claims he feels well. - Correct Answers -True, yes you can!
The body temp does not exceed 37.5 degrees C.
True or False: Hepatitis B is cause for permanent deferral? - Correct Answers -True,
hepatitis or any liver condition is cause for permanent deferral.
What is the minimum age for blood donation? - Correct Answers -16 (but it also varies
by state), Go with 16.
What is the minimum Hct for blood donation? - Correct Answers -38 Hct.
What is the maximum body temperature a person can have and still donate blood? -
Correct Answers -99.5 degrees F or 37.5 degrees C
What is the maximum range of blood pressure that a person is still able to donate
blood? - Correct Answers -May not exceed 180/100 (S/D)
True or false: Vaccines from dead microbial sources are cause for deferral for blood
donation? - Correct Answers -False. People who have synthetic derivatives or biological
toxoids or dead pathogens are allowed to donate.
True or false: A woman who had a spontaneous abortion 2 months prior is allowed to
donate blood? - Correct Answers -True. This female patient is allowed to donate blood.
Prior to donating blood, what compound should you use to prepare the venous puncture
site? - Correct Answers -Iodine compounds
What is the minimum PLT count to donate PLTs? - Correct Answers -150 x 10^3 (150 x
10^9/L)
What serologic test must be performed on all blood donations? - Correct Answers -
Serologic Test for syphilis
,In PLT preparation from a band-new donor unit, do you warm the unit or cool the unit
during PLT concentration, (extraction). - Correct Answers -You cool the unit to ~ 20-24
degrees C.
What is the most common cause for post transfusion hepatitis? - Correct Answers -
Hepatitis B, HBs Ag.
What laboratory molecular method do we use to confirm HIV in a patient? - Correct
Answers -Western Blot using patient HIV anti-HIV-1 antibodies from his or her serum.
What is the total test used to detect HIV in a patient? - Correct Answers -Serologic tests
for antibodies against HIV and 1 and 2
What is screening test used for HIV detection? - Correct Answers -ELISA test for HIV.
Rejuvenation of a unit of RBCs does what to the donor unit? - Correct Answers -It
restores the 2,3 DPG and ATP to normal levels
True or False: Splitting of a donor RBC unit under sterile and closed conditions, will
cause the donor unit to hold its original expiration date? - Correct Answers -True.
Splitting of a unit under sterile and closed conditions won't breach the unit. The
expiration date is held as original.
A donor unit is split for a pediatric patient into multiple bags. A pediatric quad set was
used. If the original donor unit had an expiration date 35 days from now, what will the
new expiration of peds bags and donor unit be? - Correct Answers -Both will have an
expiration of 35 days from now. We used a closed sterilized device to transfer. In total,
the original expiration of the bag holds true!
A donor unit was split using a closed system at 0800 and has an expiration date of
12/01/2015. Today's date is 11/06/2015. What is the expiration of the split unit? -
Correct Answers -12/01/2015. The same date because the donor unit wasn't breached
and remains sterile.
What is the optimum temperature for frozen RBCs? Why? - Correct Answers --80
degrees C. You're in a cryostat which causes amphorpic crystals to form, water to be
removed in a highly regulated manner, which in total does not lyse all the cells.
A donor unit is issued to the cardiac unit at 0900. The unit is returned at 0910. The unit
was not breached or used. What is the best course of action and why? - Correct
Answers -Put the donor unit back on the shelf in the RBC fridge. It's not breached, still
sterile. It's expiration date holds true as the original outgoing unit.
What is the optimum temperature for storing RBC donor units? - Correct Answers -4
degrees C
, A RBC donor unit was slit in an open system using graduated cylinders at 0934. The
expiration date on the unit is 12/01/2015. Today is 11/06/2015. What is the expiration
date of the new unit? Give me the date. - Correct Answers -The expiration date is 24 hrs
post preparation of a breached unit. The new expiration date is 0934 11/07/2015.
What is the optimum storage temp for cryo AHF? - Correct Answers --20 degrees C.
What is the minimum storage temp for cyro AHF? - Correct Answers --18 degrees C.
After thawing cryo AHF. How much time do you have to transfuse the patient? - Correct
Answers -4 hours. If you go over 4 hours you have to waste the product.
What is the shelf life for cyro AHF in a frozen state? - Correct Answers -12 month / 1
year
After having thawed FFP, how much time do you have to infuse the patient with? -
Correct Answers -24 hours
Irradiation of donor RBC prevents replication of what WBC type? - Correct Answers -
Lymphocytes. All HTRs are correlated through CD4 Th cells, a lymphocyte. If you kill it.
You will have NO response.
What is an important determinant of PLT viability following storage? - Correct Answers -
plasma Ph
Patients who demonstrate bombay blood phenotype have what antibody present in his
or her blood? - Correct Answers -Anti-H anti-body
What antibodies is neutralized by pooled human plasma? why? - Correct Answers -anti-
Ch
anti-Ch and anti-Rg react at IAT (immediate spin, cool phase) and reacts with C4
(component of complement).
What is the minimum pH that platelets can go to before they are considered expired? -
Correct Answers -plasma pH of 6.2 A pH greater than 6.2 has to be maintained at all
times.
In the liquid state, plasma is stored at what temperature? - Correct Answers -1-6
degrees C
Why do we have to irradiate a PLT product from a mother who is doing a donation to
her child? - Correct Answers -The mother's HLA are similar to the child, but not similar
enough. When she donates to the child, because of the lack of similarity; the child would
end up with a type I allergic response.
